Study of utilizing oil-extracted microalgae residues for butanol production by Clostridium acetobutylicum

  • 吳 書嫻

Student thesis: Master's Thesis

Abstract

Since industrial resolution fossil fuels supply most of the energy demand all over the world which will be limited in the next few decades The renewable energy becomes more and more important During biodiesel extraction the residues containing half of carbohydrate are usually remained which is the potential energy source that can be utilized Therefore this study has focused on the energy recovery from oil-extracted microalgae residues (OMR) ABE fermentation carried out by Clostridium acetobutylicum was applied to treat the OMR Bacteria could be inhibited when initial carbohydrate was higher than 30 g/L Fed-batch process could eliminate the inhibitory effect by substrate and butanol production reached 8 83 g/L Addition of 15 g/L butyrate to carbohydrate obtained the maximum butanol yield 0 192 g-butanol/g-carbohydrogen On the other hand OMR was fed to hydrogen producing bacteria to produce hydrogen and butyrate This 4 5 g/L of biological butyrate was added to ABE fermentation and promoted the butanol yield about 108% OMR was also fed to CH4 fermentation that one-stage process with 20 g/L pretreated OMR obtained the best performance The yield of H2 and CH4 were 59 mL/g-OMR and 243 mL/g-OMR respectively The one-stage process of CH4 fermentation had the maximum energy generation 10 43 kJ/g-OMR The ABE fermentation with 4 5 g/L biological butyrate addition obtained maximum energy generation rate 0 676 kJ/g-OMR/day Results indicate that Clostridium acetobutylicum can utilize OMR and butanol can be enhance by appropriate operation
Date of Award2015 Aug 3
Original languageEnglish
SupervisorLiang-Ming Whang (Supervisor)

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