Study on AC Electro-kinetics Enhanced Electrochemical Immuno-chip to Detect Cardiac Troponin I

Abstract

Myocardial infarction (MI) is mostly induced by coronary artery disease (CAD) which is one of the major lethality worldwide and it is predicted to rise Although conventional immunoassays achieve good sensitivity and specificity the long reaction time and bulky optical instruments are needed Therefore the sensing performances involving fast high sensitivity low cost and reproducibility are critical for the development of biosensors For increasing the sensitivity and decreasing the reaction time the integration of electrokinetic alternating current electro-osmosis (ACEO) into label-free electrochemical detection could facilitate antibody-antigen reaction by driving fluidic convection Furthermore a good reproducibility was achieved by optimizing the procedures of surface modification on gold electrode such as self-assembled monolayer chemical linker antibody and blocker Various electrochemical detections consisting of cyclic voltammetry (CV) square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS) were used to quantify cardiac troponin I (cTnI) which is an important indicator of MI The data showed that applying concentric electrode optimal frequency 800 Hz inner 8 Vpp and outer 15 Vpp with bias 0 5 Vpp to generate the concentrated effect which can eliminate reaction time from 15 min to 20 s and with a pause enhances the binding rate of analyte due to the re-stirring With the prospect of automation faster and more accurate detection can be achieved

Date of Award	2016 Aug 24
Original language	English
Supervisor	Hsien-Chang Chang (Supervisor)