Study on the antioxidant antigenotoxicity anti-hyperglycemia and anti-hyperlipidemia of mushrooms

  • 胡 淑惠

Student thesis: Doctoral Thesis

Abstract

The research is mainly aimed at physiological effects and fruiting efficiency of edible and medicinal mushrooms with solid-state or liquid submerged culture The extracts of fruiting body mycelium and fermented broth from Pleurotus citrinopileatus and P ferulae with solid-state and liquid submerged culture respectively were applied to study the physiological activities including antioxidant antigenotoxic antihyperglycemic and antihyperlipidemic effects in the in vivo and in vitro Moreover in the solid-state cultivation of mushroom the agrowastes partially replaced components of substrates to cultivate two Hericium spp for the exploration of biological efficiency The P citrinopileatus was cultivated under controlling environmental conditions including concentration of CO2 and illumination of different wavelength of light for studying fruiting quality The results showed EAM (a solvent extract) have the highest DPPH free radical-scavenging activity and ferric-reducing ability among six test samples in the study on the antioxidant and antigenotoxic activities of P ferulae Meanwhile the EAM exhibited the highest inhibition rate against every tested mutagen at a low concentration of 0 08 mg/mL and a high concentration of 0 3 mg/mL in each plate and it had also the highest anti-DNA-damaging activity among all test samples There may be an important relationship between the antioxidant and antihyperlipidemic activities of P citrinopileatus and the higher antioxidant and antihyperlipidemic activities of Pleurotus citrinopileatus both in vitro and in vivo were found with two test samples (EAE and MOE) Hepatic cells were less vacuolized in the MOE- and EAE-groups than in the other experimental groups The water-soluble exopolysaccharide from fermented broth of P citrinopileatus has an antihyperglycemic effect The diabetic rats fed with water-soluble polysaccharide of P citrinopileatus lost less body weight than those fed SPPC-free regular diet The fasting blood glucose levels of diabetic rats fed with SPPC were 44% lower than the negative controls This corroborates the findings of previous studies in the anti-artificial pulmonary metastastic tumor and immunoenhancing effects In the solid-state fermentation it displayed high in cultivation of H erinaceus and H laciniatum and indicated that the substitution might be a feasible proposition The optimum conditions for reaching the highest biological efficiency and yield of the two Hericium spp were at 23℃ and 90% ERH by using 500 g/kg of sugarcane bagasse to replace sawdust Besides the optimum wavelength of light illumination and atmosphere in the incubator might benefit the yield and quality of the mature fruiting body of P citrinopileatus A higher fruiting quality should be obtained with an atmospheric CO2 concentration of 0 1%–0 15% and a wavelength of light of 720 nm In conclusion the results suggest that the extracts from test mushrooms have fair bioactive functions including anti-oxidant antigenoxicity anti-hyperglycemia and anti-hyperlipidemia Besides it is contributive to fruiting quality via regulating solid-state culture conditions
Date of Award2014 Feb 13
Original languageEnglish
SupervisorSue-Joan Chang (Supervisor)

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