Study the dialogue between oral cancer cells and immune cells

  • 黃 俞瑄

Student thesis: Master's Thesis


Oral cancer is the 5th leading cause of cancer death in Taiwan Most of these patients demonstrate compromised immunity The induction of regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs) infiltration are important mechanisms for the tumor escape from immunological surveillance To further investigate the recruitment of MDSCs and/or Tregs during oral cancer progression in vivo mice were co-treated with 4-NQO and arecoline for oral cancer induction and their blood was monthly collected for flow cytometry The percentage of circulating Tregs but not MDSCs was significantly increased at all the time points during drug-induced oral cancer progression In addition to the increased FOXP3 expression in affected tongue tissues we also detected the increased infiltration of Tregs in cervical lymph nodes relative to that in control animals Since tumor-derived CCL22 is responsible for the accumulation of Tregs in certain cancer types we also detected a significant increase of CCL22 in drug-treated tongue tissues and a concordant increase of both CCL22 and FOXP3 expression in 68 oral cancer patients Kaplan-Meier survival curve analysis showed that high CCL22 patients tended to have poor clinical outcome related to those in low groups The expression of inflammatory cytokines including IL-1? and TNF-? in tumor microenvironment was one mechanism responsible for the increased CCL22 expression in oral cancer mouse model and oral cancer cells Intratumor expression of CCL22 was not only functional in mediating CCR4-expressing T cell migration through paracrine release but also regulating oral cancer proliferation and migration in an autocrine manner Although CCL22 was previously shown to recruit Tregs more studies are needed to address the mechanisms responsible for the increased CCL22 in oral cancer and its function involved in the crosstalk of oral cancer cells and Tregs during oral carcinogenesis
Date of Award2016 Aug 29
Original languageEnglish
SupervisorLi-Wha Wu (Supervisor)

Cite this