The comparison of animal test alternative test and predictive toxicology taking zebrafish (Danio rerio) and its embryo acute toxicity test and QSAR Toolbox as an example

Abstract

We often utilize animal tests in traditional toxicology such researches cost lots of money and time In addition they are not aligned with animal welfare The international trend is to develop alternative tests to reduce money and time which can reduce utilizing animals and close to animal welfare For our concern of ecotoxicology endpoint acute fish toxicity test (AFT) is a traditional animal test There are two alternative tests trying to reduce AFT test in vitro and in silico respectively For in vitro fish embryo toxicity test is announced as a standard protocol OECD TG236 For in silico one of the predictive toxicology methods is to utilize quantitative structure-activity relationship (QSAR) model In recent years our country has stricter management of chemical substances and must provide information on the (eco)toxicity of substances by the tonnage of utilization However we found that it lacks of ecotoxicology than toxicology information The objective is to validate and develop methods that can reduce the use of animal tests with zebrafish embryo alternative tests and by computer models Then the zebrafish embryo alternative tests and computer model methods are combined to provide an integrated testing strategy (ITS) of AFT test endpoint The ITS is combined by OECD QSAR Toolbox version 4 4 and OECD TG236 To develop the ITS the study considered proper experiment without any animal tests Moreover we would choose some substances operate the AFT test to validate the ITS results

Date of Award	2020
Original language	English
Supervisor	Ying-Jan Wang (Supervisor)