The development of multifunctional nanocomplexes for drug delivery and oral cancer therapy

Abstract

The incidence of oral cancer increases gradually and oral cancer is the fifth leading cause of cancer mortality in Taiwan Thus it is important to develop an effectively therapeutic approach for oral cancer The aim of this study is to develop a drug delivery system made of superparamagnetic iron oxide nanoparticles (SPIONs) covalently linked with alginate for oral cancer therapy Alginate-conjugated SPIONs (Fe3O4/alginate nanoparticles) is prepared by mixing NH3+-exposed magnetite (Fe3O4) nanoparticles and alginate followed by carbodiimide reaction The hydrodynamic diameter zeta potential and surface modification of Fe3O4/alginate nanoparticles are characterized by dynamic light scattering (DLS) and fourier transform infrared spectroscopy (FT-IR) Doxorubicin (DOX) is encapsulated into the drug delivery system by mixing DOX and Fe3O4/alginate nanoparticles solution followed by addition of the mixture into CaCl2 aqueous solution (DOX-loaded Fe3O4@Ca-alginate nanoparticles) The encapsulation efficiency and loading capacity are determined by fluorescence spectrometer with excitation wavelength of 485 nm and emission wavelength of 590 nm; release profiles of DOX-loaded Fe3O4@Ca-alginate nanoparticles in different medium are monitored from 24 hr to 7 days The hydrodynamic diameter and zeta potential of Fe3O4/alginate nanoparticles are 76 5±19 2 nm and -39 0±0 3 mV respectively and the FT-IR spectra of SPION-alginate reveals that alginates conjugate successfully to Fe3O4 nanoparticles The encapsulation efficiency and loading capacity exhibit maximum values when the weight ratio of DOX to Fe is 8:1 The release profile indicates that the cumulative DOX release percentage is highest at the cytoplasm mimicking buffer The in vitro cytotoxicity of DOX-loaded Fe3O4@Ca-alginate nanoparticles shows similar degree of cytotoxicity to that of free DOX Our results suggest that Fe3O4@Ca-alginate nanoparticles can serve as a drug nanocarrier and this system has a great potential to improve therapeutic efficiency and minimize side effects of anticancer drugs in the future

Date of Award	2014 Aug 25
Original language	English
Supervisor	Jehn-Shun Huang (Supervisor)