The Effects of Photodynamic Therapy on Bacteria Reduction for Dental Implant Application

Abstract

There is a growing popularity of dental implants in the market With the poor oral hygiene imperfect plan of pre-operation patient with smoking or periodontal disease may lead to peri-implantitis With the variety of screw shape and resistance-related bacteria photodynamic therapy (PDT) becomes a new treatment for peri-implantitis In this study two types of Gram-negative bacteria would be chose including Escherichia coli (E coli) and Porphyromonas gingivalis (P gingivalis) These bacterial were be used to research the anti-bacterial properties and combined with Toluidine Blue O (TBO) to perform photodynamic therapy First the titanium discs with different surface morphology would be prepared such as Polish SB SLA MAO and 5SrMAO to discuss the anti-bacterial properties Then we used the PDT to study the bactericidal effect of suspension biofilm system and cytotoxicity of cells From the SEM and optical density results showed that with low surface-free energy low surface roughness and surface containing strontium ion would reduce plaque accumulation in our anti-bacterial tests In the PDT experiments the results indicated that the survival rate of bacteria would decrease after PDT no matter on E coli or P gingivalis system and suspension or biofilm system Then PDT showed no significant difference between each groups of titanium discs In sum this study can conclude that strontium ion can inhibit the growth of bacterial and PDT will become a potential treatment for peri-implantitis in the future

Date of Award	2014 Aug 28
Original language	English
Supervisor	Tzer-Min Lee (Supervisor)