The Integration of Crossflow and Bioimpedance Technique in Rotating Disc Platform Design for Cell Separation

Abstract

Blood is one of the most important material that can be used to diagnose disease To prevent from the effects of blood cells for cell free plasma detection the blood separation efficiency is a critical step In this study we present a novel continuous flow separator using a cross-flow filter structure on rotating disc We combined impedance bioanalysis and a simple blood sample separation mechanism into the centrifugal platform and thus reduces the system’s complexity The working principle of the proposed separator is based on size exclusion of cell through tangential flow that induced by rotation which is different from the past rotation disc system As a pilot study of this new centrifugal flow control element we demonstrate the efficient separation that can collect the amount of the fluid without the molecules from the sample While the rotation stopped the result showed that the separation efficiency was only 96% under 3500 rpm within a short 3 seconds interval however we just can find rare particles in the analyst chamber thus we assumed that the lower efficiency may cause by the amount of fluid Though the prototype of the design had performance rapid separation the device still need to improve Therefore we conclude that the proposed device can take as reference for the application of blood separation and use for point-of-care (POC) diagnostic system

Date of Award	2017 Aug 8
Original language	English
Supervisor	Kuo-Sheng Cheng (Supervisor)