The Mechanism of Midazolam and Propofol on Mouse Leydig Cell Lines Apoptosis

  • 王 姝淳

Student thesis: Master's Thesis

Abstract

The incident rate of the testicular cancer is increasing in western world and the overall incidence rate of testicular cancer rose over time from 3 7 (per 100 000) in 1975 to 5 9 (per 100 000) in 2007 The treatment upon malignant testicular cancer is usually combined surgery with chemical drugs Midazolam and propofol are widely used as sedative and anesthetic induction agents by modulating the different GABA receptors in the central nervous system In previous study we have found that midazolam could induce MA-10 mouse Leydig tumor cell apoptosis by activating caspase cascade In addition study has shown that propofol could induce apoptosis in lung cancer through endoplasmic reticulum (ER) stress However the detail mechanisms how midazolam and propofol regulating caspase and/or ER stress pathways in Leydig tumor cells remain elusive In the present study MA-10 cells and TM3 cells (a mouse Leydig normal cell line) were used with the treatments of midazolam or propofol Results showed that cell viability significantly decreased by midazolam from 30 to 150 ?M in MA-10 cells and from 150 to 300 ?M in TM3 cells for 12 hr respectively in a dose-dependent manner (p
Date of Award2015 Jul 13
Original languageEnglish
SupervisorBu-Miin Huang (Supervisor)

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