The role of heterochromatin in Drosophila innate immune response

  • 吳 博仁

Student thesis: Master's Thesis


Innate immune system plays an important role against infections of invading pathogens Epigenetic mechanisms involving the regulation of chromatin structures which are divided into “loose” “active” euchromatin and “compact” “silent” heterochromatin states govern gene expression of physiological systems However the molecular and cellular mechanisms by which heterochromatin regulates innate immune responses remain unclear Here using Drosophila as the model system we determined whether and how heterochromatin formation mediates antimicrobial responses to the human pathogen Pseudomonas aeruginosa PA14 which is Gram-negative and highly virulent Upon PA14 infection animals with increased heterochromatin levels survived longer whereas decreasing heterochromatin exhibits shortened life span Heterochromatin levels are associated with the activity of the antimicrobial peptide (AMP) gene Drosomycin Moreover we found that pirk an important negative regulator of the Imd pathway was downregulated in flies with more heterochromatin and up-regulated in flies with less heterochromatin Taken together these data suggest that heterochromatin formation promotes innate antimicrobial responses via downregulating pirk Our work may contribute to the prevention and treatment of human infectious diseases
Date of Award2015 Aug 28
Original languageEnglish
SupervisorShian-Jang Yan (Supervisor)

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