The roles of nucleolin in Human enterovirus A infection

Abstract

Human enterovirus species A (HEV-A) is one of the four species of HEV in the genus Enterovirus in the family Picornaviridae HEV-A is known to manifest hand-food-mouth diseases in young children and can cause severe neurological diseases such as encephalitis and meningitis Among HEV-A enterovirus 71 (EV71) is the most well-known virus In our previous study we have identified nucleolin (NCL) as an EV71-interacting glycoproteins by glycoproteomic approaches NCL plays roles in EV71 binding and infection In order to understand whether NCL also involves in the binding and infection of other HEV-A viruses this study aim to investigate the roles of NCL in other HEV-A infection including coxsackievirus A2 (CVA2) CVA4 CVA6 CVA10 CVA16 Firstly we analyzed the interaction between NCL with viruses and found that all tested of 5 HEV-A viruses directly interacted with NCL by ELISA In order to verify whether NCL participates in the infection of these HEV-A viruses we developed NCL-knockdown cells We analyzed the binding and infection of the HEV-A viruses to the cells by real-time PCR immunofluorescence and flow cytometry We found that the NCL could affect the binding of CVA2 CVA6 and CVA10 to host cells and subsequently decrease the viral RNA level in the host cells However downregulation of NCL has slightly effects on the binding of CVA4 and CVA16 to the host cells The reduced cytopathic effect of CVA10 is also accompanied with NCL knockdown in RD cells These studies showed that NCL not only involved in EV71 infection but also in other HEV-A viruses infection

Date of Award	2015 Jul 29
Original language	English
Supervisor	Chuan-Fa Chang (Supervisor)