To Study the Viability of Head and Neck Cancer Cells on Non-thermal Micro-plasma-elicited ROS/RNS

Abstract

Head and neck cancer is the fifth most common cancer worldwide Anti-cancer treatment such as chemotherapy and radiotherapy is usually conducted; however the survival rates have remained virtually unchanged over the past 50 years Recent studies have shown that non-thermal micro-plasma (NTP) a physical method can heal wounds kill unexpected bacteria coagulate blood and inactivate cancer cells Plasma is a partially ionized gas containing energy particles including electrons ions and neutral atoms The effects of NTP result from reactive oxygen and nitrogen species (ROS/RNS) In this study a novel N2/He micro-plasma device is evaluated in terms of its ability to inactivate tumor cells and pharynx squamous carcinomatous cells SAS CAL 27 FaDu and Detroit 562 The temperature of NTP was measured as a function of supplied power with the addition of N2 Four cancer cell lines were subjected to N2/He micro-plasma exposure for durations of 30 60 and 90 sec A gas flow of 5 SLM (Standard Liter per Minite) He with 1% N2 for 30 60 and 90 sec was used as a positive treatment control The results indicate that NTP affected the four cell lines similarly in terms of proliferation cytotoxicity and apoptosis-related DNA damage which implies that the cell culture media plays a significant role in plasma-cell interaction SAS CAL 27 and FaDu showed a significant reduction in cell number after plasma exposure whereas Detroit 562 showed less reduction The study shows that there was a short-term effect on head and neck cancer cells with plasma exposure

Date of Award	2016 Jul 11
Original language	English
Supervisor	Jiunn-Der Liao (Supervisor)