α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

Dz Chi Chen; Bei Chang Yang; Chi Wan Chow; Tsong Teh Kuo

doi:10.1016/0168-1656(93)90064-T

α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

Dz Chi Chen, Bei Chang Yang, Chi Wan Chow, Tsong Teh Kuo

微生物學科暨微生物及免疫學研究所

研究成果: Article › 同行評審

5 引文斯高帕斯（Scopus）

摘要

We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

原文	English
頁（從 - 到）	329-334
頁數	6
期刊	Journal of Biotechnology
卷	29
發行號	3
DOIs	https://doi.org/10.1016/0168-1656(93)90064-T
出版狀態	Published - 1993 6月

All Science Journal Classification (ASJC) codes

生物技術
生物工程
應用微生物與生物技術

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10.1016/0168-1656(93)90064-T

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abstract = "We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.",

author = "Chen, {Dz Chi} and Yang, {Bei Chang} and Chow, {Chi Wan} and Kuo, {Tsong Teh}",

note = "Funding Information: Thanks are due to Drs J.R. Mattoon and R. Wu for kindly giving us the plasmids DNA. This work was supported by the National Science Council, Taiwan.",

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AU - Chow, Chi Wan

AU - Kuo, Tsong Teh

N1 - Funding Information: Thanks are due to Drs J.R. Mattoon and R. Wu for kindly giving us the plasmids DNA. This work was supported by the National Science Council, Taiwan.

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N2 - We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

AB - We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

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α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

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