α-difluoromethylornithine blocks thymocyte apoptosis via a reduction in tyrosine phosphorylation

M. S. Jan, L. Y.C. Wing, M. T. Lin, Y. S. Lin

研究成果: Article

5 引文 斯高帕斯(Scopus)

摘要

The effect of α-difluoromethylornithine on cell apoptosis was investigated. Freshly isolated mouse thymocytes were cultured in the medium alone or with dexamethasone, and apoptotic cell death was monitored after 6 h. A correlation was seen between cell apoptosis and a reduction in the polyamine levels of thymocytes. Addition of exogenous polyamines decreased the levels of apoptosis induced spontaneously in the culture medium or by dexamethasone. However, addition of α-difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase, to the cultures did not enhance apoptosis but rather caused inhibition of thymocyte apoptosis. Analysis of the mechanism of α-difluoromethylornithine-mediated inhibition of apoptosis indicated that α-difluoromethylornithine treatment blocked protein tyrosine phosphorylation, which was elevated drastically during the first hour of thymocyte cultivation. Treatment with the phosphotyrosine phosphatase inhibitor phenylarsine oxide reversed this inhibitory effect of α-difluoromethylornithine on apoptotic cell death. Our results provide an alternative mechanism for α-difluoromethylornithine showing the inhibition of apoptosis via reduction of protein tyrosine phosphorylation.

原文English
頁(從 - 到)605-611
頁數7
期刊Scandinavian Journal of Immunology
50
發行號6
DOIs
出版狀態Published - 1999 十二月 16

All Science Journal Classification (ASJC) codes

  • Immunology

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