TY - JOUR
T1 - НАПРАВЛЕННЫЙ КОЛИЧЕСТВЕННЫЙ СКРИНИНГ МАРКЕРОВ АДЕНОКАРЦИНОМЫ ЛЁГКОГО МЕТОДОМ МОНИТОРИНГА ВЫБРАННЫХ РЕАКЦИЙ
AU - Kopylov,
AU - Tikhonova, O. V.
AU - Farafonova, T. E.
AU - Novikova, S. E.
AU - Shushkova, N. A.
AU - Shevchenko, V. E.
AU - Liao, Pao Chi
AU - Archakov, A. I.
AU - Zgoda, V. G.
N1 - Publisher Copyright:
© 2018 Russian Academy of Medical Sciences. All Rights Reserved.
PY - 2018
Y1 - 2018
N2 - In the present study, we applied selected reaction monitoring (SRM) to a group of proteins that were previously reported to be associated with lung cancer (Novikova S.E. et al. (2017) Biomeditsinskaya khimiya, 63, 181-210. [1]). Measurements were performed on 59 plasma samples. These samples included: 23 samples of plasma of patients diagnosed with lung adenocarcinoma (LAC), 11 samples of plasma of patients diagnosed with squamous cell lung carcinoma (SqCC), 25 samples of donors with no previous history of oncological diseases, and one pooled sample from each of the above group. As a result of the SRM measurements 52 proteins were detected at least in one individual plasma sample. Statistical analysis showed that there were two groups confidently differentiated by the concentration value of 8 proteins wherein 5 proteins displayed increased level (P00738, P26639, P21926, P08603, P51149) in LAC group and 3 proteins (P51884, O15162, Q8N2K0) indicated diminishing the concentration level towards the control level. Data on protein concentrations obtained for LAC and SqCC did not distinguish the samples by statistical clustering analysis. These potential biomarkers can be used for further development of methods for early diagnostics of lung cancer.
AB - In the present study, we applied selected reaction monitoring (SRM) to a group of proteins that were previously reported to be associated with lung cancer (Novikova S.E. et al. (2017) Biomeditsinskaya khimiya, 63, 181-210. [1]). Measurements were performed on 59 plasma samples. These samples included: 23 samples of plasma of patients diagnosed with lung adenocarcinoma (LAC), 11 samples of plasma of patients diagnosed with squamous cell lung carcinoma (SqCC), 25 samples of donors with no previous history of oncological diseases, and one pooled sample from each of the above group. As a result of the SRM measurements 52 proteins were detected at least in one individual plasma sample. Statistical analysis showed that there were two groups confidently differentiated by the concentration value of 8 proteins wherein 5 proteins displayed increased level (P00738, P26639, P21926, P08603, P51149) in LAC group and 3 proteins (P51884, O15162, Q8N2K0) indicated diminishing the concentration level towards the control level. Data on protein concentrations obtained for LAC and SqCC did not distinguish the samples by statistical clustering analysis. These potential biomarkers can be used for further development of methods for early diagnostics of lung cancer.
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U2 - 10.18097/PBMC20186404368
DO - 10.18097/PBMC20186404368
M3 - Article
C2 - 30135285
AN - SCOPUS:85052632669
SN - 2310-6905
VL - 64
SP - 368
EP - 375
JO - Biomeditsinskaya Khimiya
JF - Biomeditsinskaya Khimiya
IS - 4
ER -
