58-kDa microspherule protein (MSP58) is novel brahma-related gene 1 (BRG1)-associated protein that modulates p53/p21 senescence pathway

Che Chia Hsu, Yi Chao Lee, Shiu Hwa Yeh, Chang Han Chen, Chih Ching Wu, Tsui Ying Wang, Yu Nong Chen, Liang Yi Hung, Yao Wen Liu, Han Ku Chen, Yi Ting Hsiao, Wei Sheng Wang, Jen Hui Tsou, Yi Huan Tsou, Mei Hsiang Wu, Wen Chang Chang, Ding Yen Lin

研究成果: Article

26 引文 斯高帕斯(Scopus)

摘要

The nucleolar 58-kDa microspherule protein (MSP58) protein is a candidate oncogene implicated in modulating cellular proliferation and malignant transformation. In this study, we show that knocking down MSP58 expression caused aneuploidy and led to apoptosis, whereas ectopic expression of MSP58 regulated cell proliferation in a context-dependent manner. Specifically, ectopic expression of MSP58 in normal human IMR90 and Hs68 diploid fibroblasts, the H184B5F5/M10 mammary epithelial cell line, HT1080 fibrosarcoma cells, primary mouse embryonic fibroblasts, and immortalized NIH3T3 fibroblasts resulted in induction of premature senescence, an enlarged and flattened cellular morphology, and increased senescence-associated β-galactosidase activity. MSP58-driven senescence was strictly dependent on the presence of functional p53 as revealed by the fact that normal cells with p53 knockdown by specific shRNA or cells with a mutated or functionally impaired p53 pathway were effective in bypassing MSP58-induced senescence. At least two senescence mechanisms are induced by MSP58. First, MSP58 activates the DNA damage response and p53/p21 signaling pathways. Second, MSP58, p53, and the SWI/SNF chromatinremodeling subunit Brahma-related gene 1 (BRG1) form a ternary complex on the p21 promoter and collaborate to activate p21. Additionally, MSP58 protein levels increased in cells undergoing replicative senescence and stress-induced senescence. Notably, the results of analyzing expression levels of MSP58 between tumors and matched normal tissues showed significant changes (both up- and down-regulation) in its expression in various types of tumors. Our findings highlight new aspects of MSP58 in modulating cellular senescence and suggest that MSP58 has both oncogenic and tumor-suppressive properties.

原文English
頁(從 - 到)22533-22548
頁數16
期刊Journal of Biological Chemistry
287
發行號27
DOIs
出版狀態Published - 2012 六月 29

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Hsu, C. C., Lee, Y. C., Yeh, S. H., Chen, C. H., Wu, C. C., Wang, T. Y., Chen, Y. N., Hung, L. Y., Liu, Y. W., Chen, H. K., Hsiao, Y. T., Wang, W. S., Tsou, J. H., Tsou, Y. H., Wu, M. H., Chang, W. C., & Lin, D. Y. (2012). 58-kDa microspherule protein (MSP58) is novel brahma-related gene 1 (BRG1)-associated protein that modulates p53/p21 senescence pathway. Journal of Biological Chemistry, 287(27), 22533-22548. https://doi.org/10.1074/jbc.M111.335331