TY - JOUR
T1 - A 5-transcript signature for discriminating viral and bacterial etiology in pediatric pneumonia
AU - Viz-Lasheras, Sandra
AU - Gómez-Carballa, Alberto
AU - Pardo-Seco, Jacobo
AU - Bello, Xabier
AU - Rivero-Calle, Irene
AU - Dacosta, Ana Isabel
AU - Kaforou, Myrsini
AU - Habgood-Coote, Dominic
AU - Cunnington, Aubrey J.
AU - Emonts, Marieke
AU - Herberg, Jethro A.
AU - Wright, Victoria J.
AU - Carrol, Enitan D.
AU - Paulus, Stephane C.
AU - Zenz, Werner
AU - Kohlfürst, Daniela S.
AU - Van der Flier, Michiel
AU - de Groot, Ronald
AU - Schlapbach, Luregn J.
AU - Agyeman, Philipp
AU - Pollard, Andrew J.
AU - Fink, Colin
AU - Kuijpers, Taco T.
AU - Anderson, Suzanne
AU - Calvo, Cristina
AU - Martínez-Padilla, María del Carmen
AU - Pérez-Aragón, Ana
AU - Gómez-Sánchez, Esteban
AU - Valencia-Ramos, Juan
AU - Giménez-Sánchez, Francisco
AU - Alonso-Quintela, Paula
AU - Moreno-Galarraga, Laura
AU - von Both, Ulrich
AU - Pokorn, Marko
AU - Zavadska, Dace
AU - Tsolia, María
AU - Vermont, Clementien L.
AU - Moll, Henriëtte A.
AU - Levin, Michael
AU - Martinón-Torres, Federico
AU - Salas, Antonio
AU - Shen, Ching-Fen
AU - EUCLIDS,
AU - DIAMONDS,
AU - GENDRES,
AU - PERFORM consortia,
N1 - Publisher Copyright:
© 2025 The Author(s)
PY - 2025/2/21
Y1 - 2025/2/21
N2 - Pneumonia stands as the primary cause of death among children under five, yet current diagnosis methods often result in inadequate or unnecessary treatments. Our research seeks to address this gap by identifying host transcriptomic biomarkers in the blood of children with definitive viral and bacterial pneumonia. We performed RNA sequencing on 192 prospectively collected whole blood samples, including 38 controls and 154 pneumonia cases, uncovering a 5-transcript signature (genes FAM20A, BAG3, TDRD9, MXRA7, and KLF14) that effectively distinguishes bacterial from viral pneumonia (area under the curve (AUC): 0.95 [0.88–1.00]). Initial validation using combined definitive and probable cases yielded an AUC of 0.87 [0.77–0.97], while full validation in a new prospective cohort of 32 patients achieved an AUC of 0.92 [0.83–1.00]. This robust signature holds significant potential to enhance diagnostics accuracy for pediatric pneumonia, reducing diagnostic delays and unnecessary treatments and potentially transforming clinical practice.
AB - Pneumonia stands as the primary cause of death among children under five, yet current diagnosis methods often result in inadequate or unnecessary treatments. Our research seeks to address this gap by identifying host transcriptomic biomarkers in the blood of children with definitive viral and bacterial pneumonia. We performed RNA sequencing on 192 prospectively collected whole blood samples, including 38 controls and 154 pneumonia cases, uncovering a 5-transcript signature (genes FAM20A, BAG3, TDRD9, MXRA7, and KLF14) that effectively distinguishes bacterial from viral pneumonia (area under the curve (AUC): 0.95 [0.88–1.00]). Initial validation using combined definitive and probable cases yielded an AUC of 0.87 [0.77–0.97], while full validation in a new prospective cohort of 32 patients achieved an AUC of 0.92 [0.83–1.00]. This robust signature holds significant potential to enhance diagnostics accuracy for pediatric pneumonia, reducing diagnostic delays and unnecessary treatments and potentially transforming clinical practice.
UR - https://www.scopus.com/pages/publications/85215439484
UR - https://www.scopus.com/pages/publications/85215439484#tab=citedBy
U2 - 10.1016/j.isci.2025.111747
DO - 10.1016/j.isci.2025.111747
M3 - Article
AN - SCOPUS:85215439484
SN - 2589-0042
VL - 28
JO - iScience
JF - iScience
IS - 2
M1 - 111747
ER -