A bead-based immunofluorescence-assay on a microfluidic dielectrophoresis platform for rapid dengue virus detection

Edwar Iswardy, Tien Chun Tsai, Hsien Chang Chang, Tzu Chuan Ho, Guey Chuen Perng, Hsien Chang Chang

研究成果: Article同行評審

30 引文 斯高帕斯(Scopus)

摘要

The proof of concept of utilizing a microfluidic dielectrophoresis (DEP) chip was conducted to rapidly detect a dengue virus (DENV) in vitro based on the fluorescence immunosensing. The mechanism of detection was that the DEP force was employed to capture the modified beads (mouse anti-flavivirus monoclonal antibody-coated beads) in the microfluidic chip and the DENV modified with fluorescence label, as the detection target, can be then captured on the modified beads by immunoreaction. The fluorescent signal was then obtained through fluorescence microscopy, and then quantified by ImageJ freeware. The platform can accelerate an immuno-reaction time, in which the on-chip detection time was 5 min, and demonstrating an ability for DENV detection as low as 104 PFU/mL. Furthermore, the required volume of DENV samples dramatically reduced, from the commonly used ~50 µL to ~15 µL, and the chip was reusable (>50x). Overall, this platform provides a rapid detection (5 min) of the DENV with a low sample volume, compared to conventional methods. This proof of concept with regard to a microfluidic dielectrophoresis chip thus shows the potential of immunofluorescence based-assay applications to meet diagnostic needs.

原文English
頁(從 - 到)174-180
頁數7
期刊Biosensors and Bioelectronics
95
DOIs
出版狀態Published - 2017 九月 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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