摘要
ARF-like proteins (ARLs) are distinct group of members or the ARF family of Ras-related GTPases. Although ARLs are very similar in primary structure to ARFs, their functions remain unclear. We cloned mouse (m) and human (h) ARL5 cDNAs to characterize the protein products and their molecular properties. mARL5 mRNA was more abundant in liver than in other adult tissues tested. mARL5, similar to mARL4, was developmentally regulated and localized to nuclei. hARL5 interacted with importin-α through its C-terminal bipartite nuclear localization signal. When expressed in COS-7 cells, mutant hARL5(T35N), which is predicted to be GDP bound, was concentrated in nucleoli. The N-terminus of hARL5, like that of ARF, was myristoylated. Yeast two-hybrid screening and in vitro protein-interaction assays showed that hARL5(Q80L), predicted to be GTP bound, interacted with heterochromatin protein 1α (HP1α), which is known to be associated with telomeres as well as with heterochromatin, and acted as a transcriptional suppressor in mammalian cells. The interaction was reproduced in COS cells, where hARL5(Q80L) was co-immunoprecipitated with HP1α. hARL5 interaction with HP1α was dependent on the nucleotide bound, and required the MIR-like motif. Moreover, hARL5(Q80L), but not hARL5 lacking the MIR-like motif, was partly co-localized with overexpressed HP1α. Our findings suggest that developmentally regulated ARL5, with its distinctive nuclear/nucleolar localization and interaction with HP1α, may play a role(s) in nuclear dynamics and/or signaling cascades during embryonic development.
| 原文 | English |
|---|---|
| 頁（從 - 到） | 4433-4445 |
| 頁數 | 13 |
| 期刊 | Journal of Cell Science |
| 卷 | 115 |
| 發行號 | 23 |
| DOIs | |
| 出版狀態 | Published - 2002 十二月 1 |
指紋
All Science Journal Classification (ASJC) codes
- Cell Biology
引用此文
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A developmentally regulated ARF-like 5 protein (ARL5), localized to nuclei and nucleoli, interacts with heterochromatin protein 1. / Lin, Ching Y.; Li, Chun-Chun; Huang, Pei Hsin; Lee, Fang Jen S.
於: Journal of Cell Science, 卷 115, 編號 23, 01.12.2002, p. 4433-4445.研究成果: Review article
TY - JOUR
T1 - A developmentally regulated ARF-like 5 protein (ARL5), localized to nuclei and nucleoli, interacts with heterochromatin protein 1
AU - Lin, Ching Y.
AU - Li, Chun-Chun
AU - Huang, Pei Hsin
AU - Lee, Fang Jen S.
PY - 2002/12/1
Y1 - 2002/12/1
N2 - ARF-like proteins (ARLs) are distinct group of members or the ARF family of Ras-related GTPases. Although ARLs are very similar in primary structure to ARFs, their functions remain unclear. We cloned mouse (m) and human (h) ARL5 cDNAs to characterize the protein products and their molecular properties. mARL5 mRNA was more abundant in liver than in other adult tissues tested. mARL5, similar to mARL4, was developmentally regulated and localized to nuclei. hARL5 interacted with importin-α through its C-terminal bipartite nuclear localization signal. When expressed in COS-7 cells, mutant hARL5(T35N), which is predicted to be GDP bound, was concentrated in nucleoli. The N-terminus of hARL5, like that of ARF, was myristoylated. Yeast two-hybrid screening and in vitro protein-interaction assays showed that hARL5(Q80L), predicted to be GTP bound, interacted with heterochromatin protein 1α (HP1α), which is known to be associated with telomeres as well as with heterochromatin, and acted as a transcriptional suppressor in mammalian cells. The interaction was reproduced in COS cells, where hARL5(Q80L) was co-immunoprecipitated with HP1α. hARL5 interaction with HP1α was dependent on the nucleotide bound, and required the MIR-like motif. Moreover, hARL5(Q80L), but not hARL5 lacking the MIR-like motif, was partly co-localized with overexpressed HP1α. Our findings suggest that developmentally regulated ARL5, with its distinctive nuclear/nucleolar localization and interaction with HP1α, may play a role(s) in nuclear dynamics and/or signaling cascades during embryonic development.
AB - ARF-like proteins (ARLs) are distinct group of members or the ARF family of Ras-related GTPases. Although ARLs are very similar in primary structure to ARFs, their functions remain unclear. We cloned mouse (m) and human (h) ARL5 cDNAs to characterize the protein products and their molecular properties. mARL5 mRNA was more abundant in liver than in other adult tissues tested. mARL5, similar to mARL4, was developmentally regulated and localized to nuclei. hARL5 interacted with importin-α through its C-terminal bipartite nuclear localization signal. When expressed in COS-7 cells, mutant hARL5(T35N), which is predicted to be GDP bound, was concentrated in nucleoli. The N-terminus of hARL5, like that of ARF, was myristoylated. Yeast two-hybrid screening and in vitro protein-interaction assays showed that hARL5(Q80L), predicted to be GTP bound, interacted with heterochromatin protein 1α (HP1α), which is known to be associated with telomeres as well as with heterochromatin, and acted as a transcriptional suppressor in mammalian cells. The interaction was reproduced in COS cells, where hARL5(Q80L) was co-immunoprecipitated with HP1α. hARL5 interaction with HP1α was dependent on the nucleotide bound, and required the MIR-like motif. Moreover, hARL5(Q80L), but not hARL5 lacking the MIR-like motif, was partly co-localized with overexpressed HP1α. Our findings suggest that developmentally regulated ARL5, with its distinctive nuclear/nucleolar localization and interaction with HP1α, may play a role(s) in nuclear dynamics and/or signaling cascades during embryonic development.
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U2 - 10.1242/jcs.00123
DO - 10.1242/jcs.00123
M3 - Review article
C2 - 12414990
AN - SCOPUS:0036903977
VL - 115
SP - 4433
EP - 4445
JO - Journal of Cell Science
JF - Journal of Cell Science
SN - 0021-9533
IS - 23
ER -