摘要
This paper presents the design and fabrication of a novel single-cell trapping dielectrophoretic (DEP) biochip, which consist of arrayed electrodes and 3D microstructures. The DEP biochips consist of ITO top electrodes, PDMS flow chambers, bottom electrode arrays, and SU-8 3D microstructure arrays. In order to achieve single-cell resolution, we fabricate a chess-type bottom electrode array and a bowl-type 3D microstructure array using excimer laser micromachining technique. The 3D structure not only yields a non-uniform electric field for DEP trapping but also enhances the positioning and immobilization of trapped cells. Theoretically, finite element method is applied to simulate the DEP forces. Experimentally, a new image processing method is developed to derive the flow dragging force on beads subjected to DEP force, and therefore estimate the magnitude of DEP force. It is shown that the chip can trap beads and cells in properly chosen media. We also fractionate beads of different sizes by bowl shaped microstructure. The proposed DEP chips have great potentials for measuring cell-membrane impendence and gene transfer in the future.
| 原文 | English |
|---|---|
| 主出版物標題 | Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 |
| 頁面 | 528-531 |
| 頁數 | 4 |
| DOIs | |
| 出版狀態 | Published - 2007 八月 28 |
| 事件 | 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 - Bangkok, Thailand 持續時間: 2007 一月 16 → 2007 一月 19 |
出版系列
| 名字 | Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 |
|---|
Other
| Other | 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 |
|---|---|
| 國家 | Thailand |
| 城市 | Bangkok |
| 期間 | 07-01-16 → 07-01-19 |
指紋
All Science Journal Classification (ASJC) codes
- Computer Networks and Communications
- Computer Science Applications
- Electrical and Electronic Engineering
引用此文
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A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures. / Wang, Kai Hsuan; Chang, Fu Ting; Lee, Yung-Chun.
Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007. 2007. p. 528-531 4160377 (Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007).研究成果: Conference contribution
TY - GEN
T1 - A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures
AU - Wang, Kai Hsuan
AU - Chang, Fu Ting
AU - Lee, Yung-Chun
PY - 2007/8/28
Y1 - 2007/8/28
N2 - This paper presents the design and fabrication of a novel single-cell trapping dielectrophoretic (DEP) biochip, which consist of arrayed electrodes and 3D microstructures. The DEP biochips consist of ITO top electrodes, PDMS flow chambers, bottom electrode arrays, and SU-8 3D microstructure arrays. In order to achieve single-cell resolution, we fabricate a chess-type bottom electrode array and a bowl-type 3D microstructure array using excimer laser micromachining technique. The 3D structure not only yields a non-uniform electric field for DEP trapping but also enhances the positioning and immobilization of trapped cells. Theoretically, finite element method is applied to simulate the DEP forces. Experimentally, a new image processing method is developed to derive the flow dragging force on beads subjected to DEP force, and therefore estimate the magnitude of DEP force. It is shown that the chip can trap beads and cells in properly chosen media. We also fractionate beads of different sizes by bowl shaped microstructure. The proposed DEP chips have great potentials for measuring cell-membrane impendence and gene transfer in the future.
AB - This paper presents the design and fabrication of a novel single-cell trapping dielectrophoretic (DEP) biochip, which consist of arrayed electrodes and 3D microstructures. The DEP biochips consist of ITO top electrodes, PDMS flow chambers, bottom electrode arrays, and SU-8 3D microstructure arrays. In order to achieve single-cell resolution, we fabricate a chess-type bottom electrode array and a bowl-type 3D microstructure array using excimer laser micromachining technique. The 3D structure not only yields a non-uniform electric field for DEP trapping but also enhances the positioning and immobilization of trapped cells. Theoretically, finite element method is applied to simulate the DEP forces. Experimentally, a new image processing method is developed to derive the flow dragging force on beads subjected to DEP force, and therefore estimate the magnitude of DEP force. It is shown that the chip can trap beads and cells in properly chosen media. We also fractionate beads of different sizes by bowl shaped microstructure. The proposed DEP chips have great potentials for measuring cell-membrane impendence and gene transfer in the future.
UR - http://www.scopus.com/inward/record.url?scp=34548143357&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548143357&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2007.352074
DO - 10.1109/NEMS.2007.352074
M3 - Conference contribution
AN - SCOPUS:34548143357
SN - 1424406102
SN - 9781424406104
T3 - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
SP - 528
EP - 531
BT - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
ER -