A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures

Kai Hsuan Wang, Fu Ting Chang, Yung-Chun Lee

研究成果: Conference contribution

2 引文 (Scopus)

摘要

This paper presents the design and fabrication of a novel single-cell trapping dielectrophoretic (DEP) biochip, which consist of arrayed electrodes and 3D microstructures. The DEP biochips consist of ITO top electrodes, PDMS flow chambers, bottom electrode arrays, and SU-8 3D microstructure arrays. In order to achieve single-cell resolution, we fabricate a chess-type bottom electrode array and a bowl-type 3D microstructure array using excimer laser micromachining technique. The 3D structure not only yields a non-uniform electric field for DEP trapping but also enhances the positioning and immobilization of trapped cells. Theoretically, finite element method is applied to simulate the DEP forces. Experimentally, a new image processing method is developed to derive the flow dragging force on beads subjected to DEP force, and therefore estimate the magnitude of DEP force. It is shown that the chip can trap beads and cells in properly chosen media. We also fractionate beads of different sizes by bowl shaped microstructure. The proposed DEP chips have great potentials for measuring cell-membrane impendence and gene transfer in the future.

原文English
主出版物標題Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
頁面528-531
頁數4
DOIs
出版狀態Published - 2007 八月 28
事件2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 - Bangkok, Thailand
持續時間: 2007 一月 162007 一月 19

出版系列

名字Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007

Other

Other2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
國家Thailand
城市Bangkok
期間07-01-1607-01-19

指紋

Biochips
Microstructure
Electrodes
Gene transfer
Micromachining
Excimer lasers
Cell membranes
Image processing
Electric fields
Finite element method
Fabrication

All Science Journal Classification (ASJC) codes

  • Computer Networks and Communications
  • Computer Science Applications
  • Electrical and Electronic Engineering

引用此文

Wang, K. H., Chang, F. T., & Lee, Y-C. (2007). A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures. 於 Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 (頁 528-531). [4160377] (Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007). https://doi.org/10.1109/NEMS.2007.352074
Wang, Kai Hsuan ; Chang, Fu Ting ; Lee, Yung-Chun. / A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures. Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007. 2007. 頁 528-531 (Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007).
@inproceedings{c0bb2238c72e4c37913c08db179e90e2,
title = "A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures",
abstract = "This paper presents the design and fabrication of a novel single-cell trapping dielectrophoretic (DEP) biochip, which consist of arrayed electrodes and 3D microstructures. The DEP biochips consist of ITO top electrodes, PDMS flow chambers, bottom electrode arrays, and SU-8 3D microstructure arrays. In order to achieve single-cell resolution, we fabricate a chess-type bottom electrode array and a bowl-type 3D microstructure array using excimer laser micromachining technique. The 3D structure not only yields a non-uniform electric field for DEP trapping but also enhances the positioning and immobilization of trapped cells. Theoretically, finite element method is applied to simulate the DEP forces. Experimentally, a new image processing method is developed to derive the flow dragging force on beads subjected to DEP force, and therefore estimate the magnitude of DEP force. It is shown that the chip can trap beads and cells in properly chosen media. We also fractionate beads of different sizes by bowl shaped microstructure. The proposed DEP chips have great potentials for measuring cell-membrane impendence and gene transfer in the future.",
author = "Wang, {Kai Hsuan} and Chang, {Fu Ting} and Yung-Chun Lee",
year = "2007",
month = "8",
day = "28",
doi = "10.1109/NEMS.2007.352074",
language = "English",
isbn = "1424406102",
series = "Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007",
pages = "528--531",
booktitle = "Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007",

}

Wang, KH, Chang, FT & Lee, Y-C 2007, A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures. 於 Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007., 4160377, Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007, 頁 528-531, 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007, Bangkok, Thailand, 07-01-16. https://doi.org/10.1109/NEMS.2007.352074

A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures. / Wang, Kai Hsuan; Chang, Fu Ting; Lee, Yung-Chun.

Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007. 2007. p. 528-531 4160377 (Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007).

研究成果: Conference contribution

TY - GEN

T1 - A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures

AU - Wang, Kai Hsuan

AU - Chang, Fu Ting

AU - Lee, Yung-Chun

PY - 2007/8/28

Y1 - 2007/8/28

N2 - This paper presents the design and fabrication of a novel single-cell trapping dielectrophoretic (DEP) biochip, which consist of arrayed electrodes and 3D microstructures. The DEP biochips consist of ITO top electrodes, PDMS flow chambers, bottom electrode arrays, and SU-8 3D microstructure arrays. In order to achieve single-cell resolution, we fabricate a chess-type bottom electrode array and a bowl-type 3D microstructure array using excimer laser micromachining technique. The 3D structure not only yields a non-uniform electric field for DEP trapping but also enhances the positioning and immobilization of trapped cells. Theoretically, finite element method is applied to simulate the DEP forces. Experimentally, a new image processing method is developed to derive the flow dragging force on beads subjected to DEP force, and therefore estimate the magnitude of DEP force. It is shown that the chip can trap beads and cells in properly chosen media. We also fractionate beads of different sizes by bowl shaped microstructure. The proposed DEP chips have great potentials for measuring cell-membrane impendence and gene transfer in the future.

AB - This paper presents the design and fabrication of a novel single-cell trapping dielectrophoretic (DEP) biochip, which consist of arrayed electrodes and 3D microstructures. The DEP biochips consist of ITO top electrodes, PDMS flow chambers, bottom electrode arrays, and SU-8 3D microstructure arrays. In order to achieve single-cell resolution, we fabricate a chess-type bottom electrode array and a bowl-type 3D microstructure array using excimer laser micromachining technique. The 3D structure not only yields a non-uniform electric field for DEP trapping but also enhances the positioning and immobilization of trapped cells. Theoretically, finite element method is applied to simulate the DEP forces. Experimentally, a new image processing method is developed to derive the flow dragging force on beads subjected to DEP force, and therefore estimate the magnitude of DEP force. It is shown that the chip can trap beads and cells in properly chosen media. We also fractionate beads of different sizes by bowl shaped microstructure. The proposed DEP chips have great potentials for measuring cell-membrane impendence and gene transfer in the future.

UR - http://www.scopus.com/inward/record.url?scp=34548143357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548143357&partnerID=8YFLogxK

U2 - 10.1109/NEMS.2007.352074

DO - 10.1109/NEMS.2007.352074

M3 - Conference contribution

AN - SCOPUS:34548143357

SN - 1424406102

SN - 9781424406104

T3 - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007

SP - 528

EP - 531

BT - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007

ER -

Wang KH, Chang FT, Lee Y-C. A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures. 於 Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007. 2007. p. 528-531. 4160377. (Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007). https://doi.org/10.1109/NEMS.2007.352074