A dielectrophoretic single-cell trapping chip with multiple electrodes and arrayed 3D microstructures

Kai Hsuan Wang, Fu Ting Chang, Yung-Chun Lee

研究成果: Conference contribution

2 引文 斯高帕斯(Scopus)

摘要

This paper presents the design and fabrication of a novel single-cell trapping dielectrophoretic (DEP) biochip, which consist of arrayed electrodes and 3D microstructures. The DEP biochips consist of ITO top electrodes, PDMS flow chambers, bottom electrode arrays, and SU-8 3D microstructure arrays. In order to achieve single-cell resolution, we fabricate a chess-type bottom electrode array and a bowl-type 3D microstructure array using excimer laser micromachining technique. The 3D structure not only yields a non-uniform electric field for DEP trapping but also enhances the positioning and immobilization of trapped cells. Theoretically, finite element method is applied to simulate the DEP forces. Experimentally, a new image processing method is developed to derive the flow dragging force on beads subjected to DEP force, and therefore estimate the magnitude of DEP force. It is shown that the chip can trap beads and cells in properly chosen media. We also fractionate beads of different sizes by bowl shaped microstructure. The proposed DEP chips have great potentials for measuring cell-membrane impendence and gene transfer in the future.

原文English
主出版物標題Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
頁面528-531
頁數4
DOIs
出版狀態Published - 2007 8月 28
事件2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 - Bangkok, Thailand
持續時間: 2007 1月 162007 1月 19

出版系列

名字Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007

Other

Other2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
國家/地區Thailand
城市Bangkok
期間07-01-1607-01-19

All Science Journal Classification (ASJC) codes

  • 電腦網路與通信
  • 電腦科學應用
  • 電氣與電子工程

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