A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals

Chia Hsien Yeh, Yu Huai Chang, Hong Ping Lin, Tsung Chain Chang, Yu Cheng Lin

研究成果: Conference contribution

摘要

This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, we discuss the probe specific test for different species of bacteria, for same species but different strains, and for same genus but different species.

原文English
主出版物標題Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
發行者Chemical and Biological Microsystems Society
頁面1004-1007
頁數4
ISBN(列印)9780979806421
出版狀態Published - 2009 1月 1
事件13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
持續時間: 2009 11月 12009 11月 5

出版系列

名字Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
國家/地區Korea, Republic of
城市Jeju
期間09-11-0109-11-05

All Science Journal Classification (ASJC) codes

  • 化學工程(雜項)
  • 生物工程

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