A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals

C. H. Yeh; Y. H. Chang; H. P. Lin; T. C. Chang; Y. C. Lin

doi:10.1016/j.proche.2009.07.083

A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals

C. H. Yeh, Y. H. Chang, H. P. Lin, T. C. Chang, Y. C. Lin

研究成果: Conference article › 同行評審

2 引文斯高帕斯（Scopus）

摘要

This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, the probe specific tests are discussed for different species of bacteria, for same species but different strains, and for same genus but different species.

原文	English
頁（從 - 到）	333-336
頁數	4
期刊	Procedia Chemistry
卷	1
發行號	1
DOIs	https://doi.org/10.1016/j.proche.2009.07.083
出版狀態	Published - 2009 9月
事件	Eurosensors 23rd Conference - Lausanne, Switzerland 持續時間: 2009 9月 6 → 2009 9月 9

All Science Journal Classification (ASJC) codes

一般化學
一般化學工程

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10.1016/j.proche.2009.07.083

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title = "A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals",

abstract = "This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, the probe specific tests are discussed for different species of bacteria, for same species but different strains, and for same genus but different species.",

author = "Yeh, {C. H.} and Chang, {Y. H.} and Lin, {H. P.} and Chang, {T. C.} and Lin, {Y. C.}",

year = "2009",

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language = "English",

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AU - Yeh, C. H.

AU - Chang, Y. H.

AU - Lin, H. P.

AU - Chang, T. C.

AU - Lin, Y. C.

PY - 2009/9

Y1 - 2009/9

N2 - This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, the probe specific tests are discussed for different species of bacteria, for same species but different strains, and for same genus but different species.

AB - This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, the probe specific tests are discussed for different species of bacteria, for same species but different strains, and for same genus but different species.

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DO - 10.1016/j.proche.2009.07.083

M3 - Conference article

AN - SCOPUS:71549149007

SN - 1876-6196

VL - 1

SP - 333

EP - 336

JO - Procedia Chemistry

JF - Procedia Chemistry

IS - 1

T2 - Eurosensors 23rd Conference

Y2 - 6 September 2009 through 9 September 2009

ER -

A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals

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