A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

Li Ying Hsieh, Hsiu Hui Chan, Ping Chung Kuo, Hsin Yi Hung, Yue Chiun Li, Chao Lin Kuo, Yong Peng, Zhong Zhen Zhao, Daih Huang Kuo, I. Wen Sun, Tian Shung Wu

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9 引文 斯高帕斯(Scopus)

摘要

Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

原文English
頁(從 - 到)1105-1112
頁數8
期刊Journal of Food and Drug Analysis
26
發行號3
DOIs
出版狀態Published - 2018 七月

All Science Journal Classification (ASJC) codes

  • Food Science
  • Pharmacology

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