A gene delivery system based on the N-terminal domain of human topoisomerase I

Yi An Chen, Hsiao Che Kuo, Young Mao Chen, Shin Yi Huang, Yu Ru Liu, Su Ching Lin, Huey Lang Yang, Tzong Yueh Chen

研究成果: Article

2 引文 斯高帕斯(Scopus)

摘要

The N-terminal 200 amino acid residues of topoisomerase I (TopoN) is highly positive in charge and has DNA binding activity, without DNA sequence and topological specificity. Here, a fusion protein (6 × His-PTD-TopoN) containing a hexahistidine (6 × His) tag, a membrane penetration domain and TopoN (amino acid 3-200) was designed and developed. The protein can bind to different sizes (3.0-8.0 kb) and forms (circular and linear) of DNA and translocates the bound DNA to the nucleus. The protein also showed low cytotoxicity to GF-1 grouper fish fin cells that were previously very sensitive and difficult to transfect in vitro. Maintaining the hexahistidine tag increased the protein's transfection efficiency in COS7 African green monkey kidney cells and simplified the purification process. The plasmid pEGFP-N1 was delivered into COS7 cells by the protein in ATP- and temperature-dependent manners. The results indicate that the binding ability of TopoN is very useful for DNA delivery and the carrier protein can be expressed in Escherichia coli without removal of the hexahistidine tag.

原文English
頁(從 - 到)4174-4184
頁數11
期刊Biomaterials
32
發行號17
DOIs
出版狀態Published - 2011 六月 1

    指紋

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

引用此

Chen, Y. A., Kuo, H. C., Chen, Y. M., Huang, S. Y., Liu, Y. R., Lin, S. C., Yang, H. L., & Chen, T. Y. (2011). A gene delivery system based on the N-terminal domain of human topoisomerase I. Biomaterials, 32(17), 4174-4184. https://doi.org/10.1016/j.biomaterials.2011.02.041