A high-throughput and quantitative hierarchical oligonucleotide primer extension (HOPE)-based approach to identify sources of faecal contamination in water bodies

Pei Ying Hong, Jer Horng Wu, Wen Tso Liu

研究成果: Article同行評審

24 引文 斯高帕斯(Scopus)

摘要

As faecal contamination of recreational and drinking water impairs the water quality and threatens public health, water bodies are routinely monitored for faecal coliforms to detect contamination. However, faecal coliforms are facultative anaerobes that survive and reproduce in ambient waters, and their presence does not depict the origin of contamination. Therefore, the use of Bacteroides-Prevotella 16S rRNA gene to perform faecal source tracking has been proposed and applied. Here, we demonstrate the use of a new molecular method termed hierarchical oligonucleotide primer extension (HOPE) to simultaneously detect human-associated Bacteroides spp. and three clusters of cow-, pig- and dog-specific uncultivated Bacteroidales. The method correctly identifies the origin of faecal contamination when tested against human, cow, pig and dog faeces (n = 17, 17, 16 and 13 respectively), and in waters contaminated with faeces of known origins. Subsequent tests with a total of 21 blind samples show that HOPE is able to accurately indicate single or multiple sources of faecal contamination originating from pigs, cows and humans in 81% of the blind samples. HOPE can further correctly detect and identify faecal contamination in five sampling sites located along a canal in southern Taiwan, and the results are validated against conventional faecal coliform tests and quantitative PCR. Overall, this study demonstrates HOPE as a quantitative and high-throughput method that can identify sources of faecal contamination.

原文English
頁(從 - 到)1672-1681
頁數10
期刊Environmental Microbiology
11
發行號7
DOIs
出版狀態Published - 2009 7月

All Science Journal Classification (ASJC) codes

  • 微生物學
  • 生態學、進化論、行為學與系統學

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