摘要
A novel microflow cytometer is proposed in which the particles are focused in the horizontal and vertical directions by means of the Saffman shear lift force generated within a micro-weir microchannel. The proposed device is fabricated on stress-relieved glass substrates and is characterized both numerically and experimentally using fluorescent particles with diameters of 5 μm and 10 μm, respectively. The numerical results show that the micro-weir structures confine the particle stream to the center of the microchannel without the need for a shear flow. Moreover, the experimental results show that the particles emerging from the micro-weir microchannel pass through the detection region in a one-by-one fashion. The focusing effect of the micro-weir microchannel is quantified by computing the normalized variance of the optical detection signal intensity. It is shown that the focusing performance of the micro-weir structure is equal to 99.% and 99.57% for the 5-μm and 10-μm beads, respectively. Overall, the results presented in this study confirm that the proposed microcytometer enables the reliable sorting and counting of particles with different diameters.
原文 | English |
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文章編號 | 034110 |
期刊 | Biomicrofluidics |
卷 | 6 |
發行號 | 3 |
DOIs | |
出版狀態 | Published - 2012 7月 18 |
All Science Journal Classification (ASJC) codes
- 分子生物學
- 材料科學(全部)
- 遺傳學
- 凝聚態物理學
- 物理與理論化學