A lab-use microfluidic planar patch-clamp system

Ting-Yuan Tu, Chang Yu Chen, De Shien Jong, Andrew M. Wo

研究成果: Conference contribution

摘要

This work presents a microfluidic planar patch-clamp system suitable for laboratory usage without high entry threshold as the traditional pipette technique. Our previously developed hourglass-shaped aperture integrated with simple mi-crofluidic system available for whole-cell recording on HIT-T15, CHO-K1, and HEK-293T cell lines. Endogenous outward rectifier and A-type potassium currents were measured from HEK-293T. Extracellular solution are exchangeable up to six different solutions for ion channel interrogation. The fastest extracellular solution exchange rate demonstrated on HEK cells was within 500ms. The lab-use microfluidic planar patch-clamp system is believed to serve as a practical mi-crodevice for general laboratory usage.

原文English
主出版物標題14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
頁面929-931
頁數3
出版狀態Published - 2010 十二月 1
事件14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
持續時間: 2010 十月 32010 十月 7

出版系列

名字14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
國家Netherlands
城市Groningen
期間10-10-0310-10-07

指紋

Clamping devices
Microfluidics
Potassium
Ion exchange
Cells
Ions

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

引用此文

Tu, T-Y., Chen, C. Y., Jong, D. S., & Wo, A. M. (2010). A lab-use microfluidic planar patch-clamp system. 於 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (頁 929-931). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; 卷 2).
Tu, Ting-Yuan ; Chen, Chang Yu ; Jong, De Shien ; Wo, Andrew M. / A lab-use microfluidic planar patch-clamp system. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. 頁 929-931 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).
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Tu, T-Y, Chen, CY, Jong, DS & Wo, AM 2010, A lab-use microfluidic planar patch-clamp system. 於 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, 卷 2, 頁 929-931, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10-10-03.

A lab-use microfluidic planar patch-clamp system. / Tu, Ting-Yuan; Chen, Chang Yu; Jong, De Shien; Wo, Andrew M.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 929-931 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; 卷 2).

研究成果: Conference contribution

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Tu T-Y, Chen CY, Jong DS, Wo AM. A lab-use microfluidic planar patch-clamp system. 於 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 929-931. (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).