A lab-use microfluidic planar patch-clamp system

Ting Yuan Tu, Chang Yu Chen, De Shien Jong, Andrew M. Wo

研究成果: Conference contribution

摘要

This work presents a microfluidic planar patch-clamp system suitable for laboratory usage without high entry threshold as the traditional pipette technique. Our previously developed hourglass-shaped aperture integrated with simple mi-crofluidic system available for whole-cell recording on HIT-T15, CHO-K1, and HEK-293T cell lines. Endogenous outward rectifier and A-type potassium currents were measured from HEK-293T. Extracellular solution are exchangeable up to six different solutions for ion channel interrogation. The fastest extracellular solution exchange rate demonstrated on HEK cells was within 500ms. The lab-use microfluidic planar patch-clamp system is believed to serve as a practical mi-crodevice for general laboratory usage.

原文English
主出版物標題14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
頁面929-931
頁數3
出版狀態Published - 2010
事件14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
持續時間: 2010 10月 32010 10月 7

出版系列

名字14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
國家/地區Netherlands
城市Groningen
期間10-10-0310-10-07

All Science Journal Classification (ASJC) codes

  • 控制與系統工程

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