@article{3decf55691954f238db50d71499b2cb1,
title = "A label-free impedimetric genosensor for the nucleic acid amplification-free detection of extracted RNA of dengue virus",
abstract = "Developing rapid and sensitive diagnostic methods for dengue virus (DENV) infection is of prime priority because DENV infection is the most prevalent mosquito-borne viral disease. This work proposes an electrochemical impedance spectroscopy (EIS)-based genosensor for the label-free and nucleic acid amplification-free detection of extracted DENV RNA intended for a sensitive diagnosis of DENV infection. A concentration ratio of 0.04 mM 6-mercaptohexanoic acid (MHA) to 1 mM 6-mercapto-1-hexanol (MCH) was selected to modify thin-film gold electrodes as a link to control the coverage of self-designed probe DNA (pDNA) at a density of 4.5 ± 0.4 × 1011 pDNA/cm2. The pDNA/MHA/MCH-modified genosensors are proven to improve the hybridization efficiency of a synthetic 160-mer target DNA (160mtDNA) with a 140-mer electrode side overhang as compared to other MHA/MCH ratio-modified genosensors. The MHA(0.04 mM)/MCH(1 mM)-modified genosensors also present good hybridization efficiency with the extracted DENV serotype 1 (DENV1) RNA samples, having the same electrode side overhangs with the 160mtDNA, showing a low detection limit of 20 plaque forming units (PFU)/mL, a linear range of 102–105 PFU/mL and good selectivity for DENV1. The pDNA density-controlled method has great promise to construct sensitive genosensors based on the hybridization of extracted DENV nucleic acids.",
author = "Wu, {Ching Chou} and Yen, {Hao Yu} and Lai, {Lu Ting} and Perng, {Guey Chuen} and Lee, {Cheng Rei} and Wu, {Shuenn Jue}",
note = "Funding Information: Funding: This research was financially supported by the Henry M. Jackson Foundation for the Advancement of Military Medicine grant number [895748]; the Naval Medical Research Center (NMRC) grant number [N3239818P0305]; the Ministry of Science and Technology of Taiwan grant number [MOST107-2313-B-005-006-MY3], and the “Innovation and Development Center of Sustainable Agriculture” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education in Taiwan. This work was partly funded by the Military Infectious Diseases Research Program grant number [L0427_16_NM] and supported by NMRC Work Unit Number A1223. Funding Information: Disclaimer: The views expressed in this article are those of the authors, and do not necessarily reflect the official policy or position of the Department of the Navy, the Department of Defense, or the US Government. Shuenn-Jue Wu is a U.S. government employee. This work was prepared as part of her official duties. Title 17 U.S.C. § 105 provides that {\textquoteleft}Copyright protection under this title is not available for any work of the United States Government.{\textquoteright} Title 17 U.S.C. § 101 defines a U.S. Government work as a work prepared by military service members or employees of the U.S. Government as part of those persons{\textquoteright} official duties. Cheng-Rei Lee was employed by the Henry M. Jackson Foundation for the Advancement of Military Medicine and was funded to do this work by the U.S. Government. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2020",
month = jul,
day = "1",
doi = "10.3390/s20133728",
language = "English",
volume = "20",
pages = "1--15",
journal = "Sensors",
issn = "1424-3210",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "13",
}