C-reactive protein (CRP) has been used as a common indicator during inflammation process. It has been also reported that CRP concentration in serum can be used for risk assessment of cardiovascular diseases. In this study, a new microfluidic system for automatic measurement of CRP is developed. When compared to our previous work, the new chip can perform the entire measurement process by integrating a new micro-injector with other functional microfluidic devices. Experimental data show that the developed system can automate the entire process within 35 minutes with a high sensitivity. The development of the new system may provide a promising platform for automatic measurement of the CRP for point-of-care applications.