A Method for Measuring Rho Kinase Activity in Tissues and Cells

Ping-Yen Liu, James K. Liao

研究成果: Chapter

47 引文 斯高帕斯(Scopus)

摘要

The Rho-associated kinases (ROCKs) can regulate cell shape and function by modulating the actin cytoskeleton. ROCKs are serine-threonine protein kinases that can phosphorylate adducin, ezrin-radixin-moesin proteins, LIM kinase, and myosin light chain phosphatase. In the cardiovascular system, the RhoA/ROCK pathway has been implicated in angiogenesis, atherosclerosis, cerebral and coronary vasospasm, cerebral ischemia, hypertension, myocardial hypertrophy, and neointima formation after vascular injury. ROCKs consist of two isoforms: ROCK1 and ROCK2. They share overall 65% homology in their amino acid sequence and 92% homology in their amino kinase domains. However, these two isoforms have different subcellular localizations and exert biologically different functions. In particular, ROCK1 appears to be more important for immunological functions, whereas ROCK2 is more important for endothelial and vascular smooth muscle function. Thus, the ability to measure ROCK activity in tissues and cells would be important for understanding mechanisms underlying cardiovascular disease. This chapter describes a method for measuring ROCK activity in peripheral blood, tissues, and cells.

原文English
主出版物標題Small GTPases in Disease, Part B
編輯William Balch, Channing Der, Alan Hall
頁面181-189
頁數9
DOIs
出版狀態Published - 2008 九月 24

出版系列

名字Methods in Enzymology
439
ISSN(列印)0076-6879

    指紋

All Science Journal Classification (ASJC) codes

  • Biochemistry

引用此

Liu, P-Y., & Liao, J. K. (2008). A Method for Measuring Rho Kinase Activity in Tissues and Cells. 於 W. Balch, C. Der, & A. Hall (編輯), Small GTPases in Disease, Part B (頁 181-189). (Methods in Enzymology; 卷 439). https://doi.org/10.1016/S0076-6879(07)00414-4