A microfluidic cell culture platform for real-time cellular imaging

Chia Chun Hsieh, Song Bin Huang, Ping-Ching Wu, Dar-Bin Shieh, Gwo Bin Lee

研究成果: Article

24 引文 (Scopus)

摘要

This study reports a new microfluidic cell culture platform for real-time, in vitro microscopic observation and evaluation of cellular functions. Microheaters, a micro temperature sensor, and micropumps are integrated into the system to achieve a self-contained, perfusion-based, cell culture microenvironment. The key feature of the platform includes a unique, ultra-thin, culture chamber with a depth of 180 μm, allowing for real-time, high-resolution cellular imaging by combining bright field and fluorescent optics to visualize nanoparticle-cell/ organelle interactions. The cell plating, culturing, harvesting and replenishing processes are performed automatically. The developed platform also enables drug screening and real-time, in situ investigation of the cellular and sub-cellular delivery process of nano vectors. The mitotic activity and the interaction between cells and the nano drug carriers (conjugated quantum dots-epirubicin) are successfully monitored in this device. This developed system could be a promising platform for a wide variety of applications such as high-throughput, cell-based studies and as a diagnostic cellular imaging system.

原文English
頁(從 - 到)903-913
頁數11
期刊Biomedical Microdevices
11
發行號4
DOIs
出版狀態Published - 2009 四月 16

指紋

Microfluidics
Cell culture
Cell Culture Techniques
Imaging techniques
Cell Communication
Temperature sensors
Plating
Imaging systems
Semiconductor quantum dots
Cellular Microenvironment
Quantum Dots
Optics
Epirubicin
Screening
Preclinical Drug Evaluations
Drug Carriers
Throughput
Diagnostic Imaging
Nanoparticles
Organelles

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

引用此文

Hsieh, Chia Chun ; Huang, Song Bin ; Wu, Ping-Ching ; Shieh, Dar-Bin ; Lee, Gwo Bin. / A microfluidic cell culture platform for real-time cellular imaging. 於: Biomedical Microdevices. 2009 ; 卷 11, 編號 4. 頁 903-913.
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A microfluidic cell culture platform for real-time cellular imaging. / Hsieh, Chia Chun; Huang, Song Bin; Wu, Ping-Ching; Shieh, Dar-Bin; Lee, Gwo Bin.

於: Biomedical Microdevices, 卷 11, 編號 4, 16.04.2009, p. 903-913.

研究成果: Article

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