A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

Wei Chun Tsai, Lien Yu Hung, Teng Yi Huang, Yen Shen Shan, Shang Cheng Hung, Gwo Bin Lee

研究成果: Conference contribution

2 引文 斯高帕斯(Scopus)

摘要

This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

原文English
主出版物標題2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
發行者Institute of Electrical and Electronics Engineers Inc.
頁面489-493
頁數5
ISBN(電子)9781509030590
DOIs
出版狀態Published - 2017 八月 25
事件12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States
持續時間: 2017 四月 92017 四月 12

出版系列

名字2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Other

Other12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
國家/地區United States
城市Los Angeles
期間17-04-0917-04-12

All Science Journal Classification (ASJC) codes

  • 電氣與電子工程
  • 機械工業
  • 電子、光磁材料
  • 儀器

指紋

深入研究「A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides」主題。共同形成了獨特的指紋。

引用此