A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

Wei Chun Tsai; Lien Yu Hung; Teng Yi Huang; Yen Shen Shan; Shang Cheng Hung; Gwo Bin Lee

doi:10.1109/NEMS.2017.8017070

A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

Wei Chun Tsai
, Lien Yu Hung
, Teng Yi Huang
, Yen Shen Shan
, Shang Cheng Hung
, Gwo Bin Lee

臨床醫學研究所

研究成果: Conference contribution

5 引文斯高帕斯（Scopus）

摘要

This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

原文	English
主出版物標題	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
發行者	Institute of Electrical and Electronics Engineers Inc.
頁面	489-493
頁數	5
ISBN（電子）	9781509030590
DOIs	https://doi.org/10.1109/NEMS.2017.8017070
出版狀態	Published - 2017 8月 25
事件	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States 持續時間: 2017 4月 9 → 2017 4月 12

出版系列

名字	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Other

Other	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
國家/地區	United States
城市	Los Angeles
期間	17-04-09 → 17-04-12

UN SDG

此研究成果有助於以下永續發展目標

SDG 3 良好的健康和福祉

All Science Journal Classification (ASJC) codes

電氣與電子工程
機械工業
電子、光磁材料
儀器

存取文件

10.1109/NEMS.2017.8017070

其它文件與鏈接

引用此

Tsai, W. C., Hung, L. Y., Huang, T. Y., Shan, Y. S., Hung, S. C., & Lee, G. B. (2017). A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. 於 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 (頁 489-493). 文章 8017070 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2017.8017070

Tsai, Wei Chun ; Hung, Lien Yu ; Huang, Teng Yi 等. / A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. 頁 489-493 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).

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title = "A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides",

abstract = "This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70\% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.",

author = "Tsai, \{Wei Chun\} and Hung, \{Lien Yu\} and Huang, \{Teng Yi\} and Shan, \{Yen Shen\} and Hung, \{Shang Cheng\} and Lee, \{Gwo Bin\}",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 ; Conference date: 09-04-2017 Through 12-04-2017",

year = "2017",

month = aug,

day = "25",

doi = "10.1109/NEMS.2017.8017070",

language = "English",

series = "2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017",

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Tsai, WC, Hung, LY, Huang, TY, Shan, YS, Hung, SC & Lee, GB 2017, A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. 於 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017., 8017070, 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Institute of Electrical and Electronics Engineers Inc., 頁 489-493, 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Los Angeles, United States, 17-04-09. https://doi.org/10.1109/NEMS.2017.8017070

A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. / Tsai, Wei Chun; Hung, Lien Yu; Huang, Teng Yi 等.
2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 489-493 8017070 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).

研究成果: Conference contribution

TY - GEN

T1 - A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

AU - Tsai, Wei Chun

AU - Hung, Lien Yu

AU - Huang, Teng Yi

AU - Shan, Yen Shen

AU - Hung, Shang Cheng

AU - Lee, Gwo Bin

PY - 2017/8/25

Y1 - 2017/8/25

N2 - This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

AB - This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

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DO - 10.1109/NEMS.2017.8017070

M3 - Conference contribution

AN - SCOPUS:85030859171

T3 - 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

SP - 489

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BT - 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Y2 - 9 April 2017 through 12 April 2017

ER -

Tsai WC, Hung LY, Huang TY, Shan YS, Hung SC, Lee GB. A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. 於 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 489-493. 8017070. (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). doi: 10.1109/NEMS.2017.8017070

A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

摘要

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Other

UN SDG

All Science Journal Classification (ASJC) codes

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