A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

Wen Bin Lee; Yin He Chen; Hsin I. Lin; Chao June Huang; Shu Chu Shiesh; Gwo Bin Lee

doi:10.1109/MEMSYS.2010.5442372

A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

Wen Bin Lee, Yin He Chen, Hsin I. Lin, Chao June Huang, Shu Chu Shiesh, Gwo Bin Lee

醫學檢驗生物技術學系

研究成果: Conference contribution

2 引文斯高帕斯（Scopus）

摘要

C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL^-1, which was superior to previous works.

原文	English
主出版物標題	MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
頁面	979-982
頁數	4
DOIs	https://doi.org/10.1109/MEMSYS.2010.5442372
出版狀態	Published - 2010 6月 1
事件	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China 持續時間: 2010 1月 24 → 2010 1月 28

出版系列

名字	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN（列印）	1084-6999

Other

Other	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
國家/地區	China
城市	Hong Kong
期間	10-01-24 → 10-01-28

All Science Journal Classification (ASJC) codes

電子、光磁材料
凝聚態物理學
機械工業
電氣與電子工程

UN SDG

此研究成果有助於以下永續發展目標

存取文件

10.1109/MEMSYS.2010.5442372

其它文件與鏈接

引用此

Lee, W. B., Chen, Y. H., Lin, H. I., Huang, C. J., Shiesh, S. C., & Lee, G. B. (2010). A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. 於 MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (頁 979-982). 文章 5442372 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442372

Lee, Wen Bin ; Chen, Yin He ; Lin, Hsin I. 等. / A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. 頁 979-982 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{9f722917668841c78f030ff09ab11192,

title = "A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein",

abstract = "C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL-1, which was superior to previous works.",

author = "Lee, {Wen Bin} and Chen, {Yin He} and Lin, {Hsin I.} and Huang, {Chao June} and Shiesh, {Shu Chu} and Lee, {Gwo Bin}",

year = "2010",

month = jun,

day = "1",

doi = "10.1109/MEMSYS.2010.5442372",

language = "English",

isbn = "9781424457649",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "979--982",

booktitle = "MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest",

note = "23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 ; Conference date: 24-01-2010 Through 28-01-2010",

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Lee, WB, Chen, YH, Lin, HI, Huang, CJ, Shiesh, SC & Lee, GB 2010, A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. 於 MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442372, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 頁 979-982, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 10-01-24. https://doi.org/10.1109/MEMSYS.2010.5442372

A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. / Lee, Wen Bin; Chen, Yin He; Lin, Hsin I. 等.
MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 979-982 5442372 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

研究成果: Conference contribution

TY - GEN

T1 - A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

AU - Lee, Wen Bin

AU - Chen, Yin He

AU - Lin, Hsin I.

AU - Huang, Chao June

AU - Shiesh, Shu Chu

AU - Lee, Gwo Bin

PY - 2010/6/1

Y1 - 2010/6/1

N2 - C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL-1, which was superior to previous works.

AB - C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL-1, which was superior to previous works.

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T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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BT - MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest

T2 - 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010

Y2 - 24 January 2010 through 28 January 2010

ER -

Lee WB, Chen YH, Lin HI, Huang CJ, Shiesh SC, Lee GB. A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. 於 MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 979-982. 5442372. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2010.5442372

A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

摘要

出版系列

Other

All Science Journal Classification (ASJC) codes

UN SDG

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