A net mold-based method of scaffold-free three-dimensional cardiac tissue creation

Yang Bai, Enoch Yeung, Cecillia Lui, Chin Siang Ong, Isaree Pitaktong, Chenyu Huang, Takahiro Inoue, Hiroshi Matsushita, Chunye Ma, Narutoshi Hibino

研究成果: Article同行評審

1 引文 斯高帕斯(Scopus)

摘要

This protocol describes a novel and easy net mold-based method to create three-dimensional (3-D) cardiac tissues without additional scaffold material. Human-induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs), human cardiac fibroblasts (HCFs), and human umbilical vein endothelial cells (HUVECs) are isolated and used to generate a cell suspension with 70% iPSC-CMs, 15% HCFs, and 15% HUVECs. They are co-cultured in an ultra-low attachment "hanging drop" system, which contains micropores for condensing hundreds of spheroids at one time. The cells aggregate and spontaneously form beating spheroids after 3 days of co-culture. The spheroids are harvested, seeded into a novel mold cavity, and cultured on a shaker in the incubator. The spheroids become a mature functional tissue approximately 7 days after seeding. The resultant multilayered tissues consist of fused spheroids with satisfactory structural integrity and synchronous beating behavior. This new method has promising potential as a reproducible and cost-effective method to create engineered tissues for the treatment of heart failure in the future.

原文English
文章編號e58252
期刊Journal of Visualized Experiments
2018
發行號138
DOIs
出版狀態Published - 2018 八月 5

All Science Journal Classification (ASJC) codes

  • 神經科學 (全部)
  • 化學工程 (全部)
  • 生物化學、遺傳與分子生物學 (全部)
  • 免疫學與微生物學 (全部)

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