A new microfluid system for mitochondrial DNA extraction and analysis

Chen Min Chang, Li Fang Chiou, Chun Che Lin, Dar Bin Shieh, Gwo Bin Lee

研究成果: Conference contribution

摘要

Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been reported. A cell lysis device, pneumatic micropumps, and an active micromixer were first used for fast extraction of mtDNA from cells. Then, the PCR and the MCE modules were used for nucleic acid amplification and separation of mtDNA. Experimental data showed that the deletion of mtDNA can be automatically detected with this approach.

原文English
主出版物標題2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
頁面193-196
頁數4
DOIs
出版狀態Published - 2011 四月 13
事件24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico
持續時間: 2011 一月 232011 一月 27

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(列印)1084-6999

Other

Other24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
國家Mexico
城市Cancun
期間11-01-2311-01-27

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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