A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood

I. Fang Cheng, Tzu Ying Chen, Yu De Lin, Wei Lung Huang, Chien Wei Liu, Wu Chou Su

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

An antibody-free approach for high purity and high throughput isolation of circulating tumor cells (CTCs) from human blood in a microfluidic chip is presented. High throughput continuous CTC isolation (>1.2 ml/hr) is achieved with a sustained dielectrophoresis (DEP) particle force normal to the continuous through-flow. The design allows continuous fractionation of cells into different downstream sub-channels based on the differences in their different critical DEP strengths/mobility on both sides of the cross-over. An enrichment factor of ∼105 and a recovery rate of ∼80% from a 0.001% CTC sample is achieved at a high throughput of 1.2 ml/hr. Compared to conventional physical methods, such as immunomagnetics-based methods and microfluidic biomarker-based methods, the isolation purity, recovery rate, throughput and viability of the proposed antibody-free chip demonstrated excellent capabilities, especially with regard to high enrichment at a high flow rate.

原文English
主出版物標題IEEE-NANO 2015 - 15th International Conference on Nanotechnology
發行者Institute of Electrical and Electronics Engineers Inc.
頁面1525-1527
頁數3
ISBN(電子)9781467381550
DOIs
出版狀態Published - 2015
事件15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 - Rome, Italy
持續時間: 2015 7月 272015 7月 30

出版系列

名字IEEE-NANO 2015 - 15th International Conference on Nanotechnology

Other

Other15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015
國家/地區Italy
城市Rome
期間15-07-2715-07-30

All Science Journal Classification (ASJC) codes

  • 製程化學與技術
  • 電氣與電子工程
  • 陶瓷和複合材料
  • 電子、光磁材料
  • 表面、塗料和薄膜

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