A novel DNA hybridization assay using gold-streptavidin, silver enhancement and a flatbed scanner

Chia Hsien Yeh, Yu Huai Chang, Hong-Ping Lin, Tsung Chain Chang, Yu-Cheng Lin

研究成果: Conference contribution

摘要

This paper reports a novel DNA hybridization detection method using gold-streptavidin (Au-streptavidin) conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to scan and detect the hybridization signal. The detection limit of DNA concentration is 8.25 ng/mL and we also differentiate the specific DNA samples. In addition, the time of the whole detection was 18 minutes, which is shorter than the conventional enzyme staining (approximately 60 minutes).

原文English
主出版物標題Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
發行者Chemical and Biological Microsystems Society
頁面948-951
頁數4
ISBN(列印)9780979806421
出版狀態Published - 2009 1月 1
事件13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
持續時間: 2009 11月 12009 11月 5

出版系列

名字Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
國家/地區Korea, Republic of
城市Jeju
期間09-11-0109-11-05

All Science Journal Classification (ASJC) codes

  • 化學工程(雜項)
  • 生物工程

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