A novel nattokinase produced by Pseudomonas sp. TKU015 using shrimp shells as substrate

San Lang Wang, Hsin J. Chen, Tzu-Wen Liang, Yung D. Lin

研究成果: Article同行評審

56 引文 斯高帕斯(Scopus)

摘要

A nattokinase was purified from the culture supernatant of Pseudomonas sp. TKU015 with shrimp shell wastes as the sole carbon/nitrogen source. The molecular masses of TKU015 nattokinase determined by SDS-PAGE and gel filtration were approximately 21 and 24 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of TKU015 nattokinase were 7, 50 °C, pH 4-11, and less than 50 °C, respectively. TKU015 nattokinase was inhibited completely by PMSF, indicating that the TKU015 nattokinase was serine protease. The results of peptide mass mapping showed that two tryptic peptides of the nattokinase were identical to a chitin binding protein from Bacillus cereus ATCC 14579 (GenBank accession number gi30020946) with 23% sequence coverage. With this method, Pseudomonas sp. TKU015 produces a nattokinase/fibrinolytic enzyme and may be considered as a new source for thrombolytic agents.

原文English
頁(從 - 到)70-76
頁數7
期刊Process Biochemistry
44
發行號1
DOIs
出版狀態Published - 2009 一月 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Bioengineering
  • Applied Microbiology and Biotechnology

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