A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

Kanako Tago; Sumiko Yonezawa; Toshihide Ohkouchi; Tomoyuki Ninomiya; Masayuki Hashimoto; Masahito Hayatsu

doi:10.1264/jsme2.21.53

A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

Kanako Tago, Sumiko Yonezawa, Toshihide Ohkouchi, Tomoyuki Ninomiya, Masayuki Hashimoto, Masahito Hayatsu

分子醫學研究所

研究成果: Article › 同行評審

15 引文斯高帕斯（Scopus）

摘要

The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. Strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.

原文	English
頁（從 - 到）	53-57
頁數	5
期刊	Microbes and Environments
卷	21
發行號	1
DOIs	https://doi.org/10.1264/jsme2.21.53
出版狀態	Published - 2006

All Science Journal Classification (ASJC) codes

生態學、進化論、行為學與系統學
土壤科學
植物科學

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10.1264/jsme2.21.53

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abstract = "The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. Strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.",

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T1 - A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

AU - Tago, Kanako

AU - Yonezawa, Sumiko

AU - Ohkouchi, Toshihide

AU - Ninomiya, Tomoyuki

AU - Hashimoto, Masayuki

AU - Hayatsu, Masahito

PY - 2006

Y1 - 2006

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AB - The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. Strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.

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A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

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