A novel real-time immunoassay approach utilizing an electro-immunosensing microchip

Min Li, Yu Cheng Lin, Ka Chu Su, Yu Tsung Wang, Tsung Chain Chang, Hong Ping Lin

研究成果: Conference contribution

摘要

This study was focused on the development of an Electro-Immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme conjugated with secondary antibody for detection, the process of electro-immunoassay was relatively simpler in comparison to the conventional enzyme-linked immunosorbent assay (ELISA). Based on the impedance variety in the frequency domain, we could successfully identify 1 ng/mL protein A with non-specific antigen as the negative control. The EIS chip had higher sensitivity and shorter assay time than the ELISA. Furthermore, the EIS chip could detect the kinematics of antibody-antigen binding.

原文English
主出版物標題TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers
頁面1620-1623
頁數4
出版狀態Published - 2005
事件13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 - Seoul, Korea, Republic of
持續時間: 2005 6月 52005 6月 9

出版系列

名字Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05
2

Other

Other13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05
國家/地區Korea, Republic of
城市Seoul
期間05-06-0505-06-09

All Science Journal Classification (ASJC) codes

  • 一般工程

指紋

深入研究「A novel real-time immunoassay approach utilizing an electro-immunosensing microchip」主題。共同形成了獨特的指紋。

引用此