A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement

Min Li, Yu Cheng Lin, Kai Chun Su, Yu Tsung Wang, Tsung Chain Chang, Hong Ping Lin

研究成果: Article同行評審

25 引文 斯高帕斯(Scopus)

摘要

This study focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme being conjugated with a secondary antibody for detection, the process of electro-immunoassay was found to be relatively simpler compared to the conventional enzyme-linked immunosorbent assay (ELISA). This novel testing method was designed using the wave impedance theory to measure the antibody-antigen recognition. Based on the phase variety in the frequency domain, the detection limit of protein A was 1 ng/mL. It was found that the antibody-antigen recognition has an obvious peak of the phase angle near 1.61 GHz. The EIS chip had higher sensitivity and a shorter assay time than the ELISA. The sensitivity of the immunoassay on the EIS chip was 100-fold higher than that of conventional enzyme-linked immunosorbent assay (ELISA). Using antibodies labelled with 13 nm gold nanoparticles (ANPs), the detection sensitivity of protein A was increased to 0.1 ng/mL. In addition, the EIS chip was capable of detecting the kinematics of antibody-antigen binding.

原文English
頁(從 - 到)451-456
頁數6
期刊Sensors and Actuators, B: Chemical
117
發行號2
DOIs
出版狀態Published - 2006 10月 12

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學

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