TY - JOUR
T1 - A portable sample concentrator on paper-based microfluidic devices
AU - Hung, Li Hsuan
AU - Wang, Hsiang Li
AU - Yang, Ruey Jen
N1 - Funding Information:
The authors gratefully acknowledge the financial support provided to this study by the Ministry of Science and Technology of Taiwan under Project Nos. MOST-102-2221-E-006-104-MY3 and MOST-104-2221-E-006-154-MY3.
Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Microfluidic paper-based analytical devices (µPADs) provide a promising solution for low-cost point-of-care diagnostic applications. However, much work remains to be done in optimizing their design and performance. Accordingly, this study investigates the preconcentration performance of µPADs comprising one, two and three convergent channels, respectively. The performance of the three devices is evaluated experimentally using fluorescein and a fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) sample with an initial concentration of 10−5 M. It is shown that the single-channel µPAD achieves a 20-fold improvement in the sample concentration in approximately 2 min. By contrast, the double- and triple-channel µPADs achieve preconcentration factors of 60- and 140-fold, respectively. Finally, a portable concentrator device is proposed. The experimental results show that a 100-fold improvement in the FITC-BSA sample concentration can be obtained in approximately 110 s given the use of four 16-V batteries, yielding a practical point-of-care diagnostic device.
AB - Microfluidic paper-based analytical devices (µPADs) provide a promising solution for low-cost point-of-care diagnostic applications. However, much work remains to be done in optimizing their design and performance. Accordingly, this study investigates the preconcentration performance of µPADs comprising one, two and three convergent channels, respectively. The performance of the three devices is evaluated experimentally using fluorescein and a fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) sample with an initial concentration of 10−5 M. It is shown that the single-channel µPAD achieves a 20-fold improvement in the sample concentration in approximately 2 min. By contrast, the double- and triple-channel µPADs achieve preconcentration factors of 60- and 140-fold, respectively. Finally, a portable concentrator device is proposed. The experimental results show that a 100-fold improvement in the FITC-BSA sample concentration can be obtained in approximately 110 s given the use of four 16-V batteries, yielding a practical point-of-care diagnostic device.
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U2 - 10.1007/s10404-016-1744-z
DO - 10.1007/s10404-016-1744-z
M3 - Article
AN - SCOPUS:84966356728
SN - 1613-4982
VL - 20
JO - Microfluidics and Nanofluidics
JF - Microfluidics and Nanofluidics
IS - 5
M1 - 80
ER -