A protocol for culturing drosophila melanogaster stage 9 egg chambers for live imaging

Mohit Prasad, Chuen-Chuen Jang, Michelle Starz-Gaiano, Mariana Melani, Denise J. Montell

研究成果: Article

106 引文 (Scopus)

摘要

This protocol describes a method for the dissection of egg chambers from intact Drosophila females and culture conditions that permit live imaging of them, with a particular emphasis on stage 9. This stage of development is characterized by oocyte growth and patterning, outer follicle cell rearrangement and migration of border cells. Although in vitro culture of egg chambers of later developmental stages has long been possible, until recently stage 9 egg chambers could only be kept alive for short periods, did not develop normally, and border cell migration failed entirely. We have established culture conditions that support overall egg chamber development including border cell migration in vitro. This protocol makes possible direct observation of molecular and cellular dynamics in both wild-type and mutant egg chambers, and opens the door to testing of pharmacological inhibitors and the use of biosensors. The entire protocol takes ˜24 h while the preparation of egg chambers for live imaging requires only 15-20 min.

原文English
頁(從 - 到)2467-2473
頁數7
期刊Nature Protocols
2
發行號10
DOIs
出版狀態Published - 2007 一月 1

指紋

Drosophila melanogaster
Ovum
Imaging techniques
Dissection
Biosensors
Cell Movement
Testing
Biosensing Techniques
Molecular Dynamics Simulation
Drosophila
Oocytes
Observation
Pharmacology
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

引用此文

Prasad, Mohit ; Jang, Chuen-Chuen ; Starz-Gaiano, Michelle ; Melani, Mariana ; Montell, Denise J. / A protocol for culturing drosophila melanogaster stage 9 egg chambers for live imaging. 於: Nature Protocols. 2007 ; 卷 2, 編號 10. 頁 2467-2473.
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A protocol for culturing drosophila melanogaster stage 9 egg chambers for live imaging. / Prasad, Mohit; Jang, Chuen-Chuen; Starz-Gaiano, Michelle; Melani, Mariana; Montell, Denise J.

於: Nature Protocols, 卷 2, 編號 10, 01.01.2007, p. 2467-2473.

研究成果: Article

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