A rapid and feasible1 H-NMR quantification method of ephedrine alkaloids in ephedra herbal preparations

Hsin Yi Hung, Shih Min Lin, Chia Ying Li, Sio Hong Lam, Yu Yi Chan, Meei Jen Liou, Po Chuen Shieh, Fu An Chen, Ping Chung Kuo, Tian Shung Wu

研究成果: Article同行評審

摘要

A highly specific and sensitive proton nuclear magnetic resonance (1 H-NMR) method has been developed for the quantification of ephedrine alkaloid derivatives in Ephedra herbal commercial prescriptions. At the region of δ 4.0 to 5.0 ppm in the1 H NMR spectrum, the characteristic signals are separated well from each other, and six analogues in total, methylephedrine (ME), ephedrine (EP), norephedrine (NE), norpseudoephedrine (NP), pseudoephedrine (PE), and methylpseudoephedrine (MP) could be identified. The quantities of these compounds are calculated by the relative ratio of the integral values of the target peak for each compound to the known concentrations of the internal standard anthracene. The present method allows for a rapid and simple quantification of ephedrine alkaloid derivatives in Ephedra-related commercial prescriptions without any preliminary purification steps and standard compounds, and accordingly it can be a powerful tool to verify different Ephedra species. In comparison to conventional chromatographic methods, the advantages of this method include the fact that no standard compounds are required, the quantification can be directly performed on the crude extracts, a better selectivity for various ephedrine alkaloid derivatives, and the fact that a very significant time-gain may be achieved.

原文English
文章編號1599
期刊Molecules
26
發行號6
DOIs
出版狀態Published - 2021 三月 2

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 化學(雜項)
  • 分子醫學
  • 藥學科學
  • 藥物發現
  • 物理與理論化學
  • 有機化學

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