A rapid electrochemical biosensor based on an AC electrokinetics enhanced immuno-reaction

I. Fang Cheng, Hsiao Lan Yang, Cheng Che Chung, Hsien Chang Chang

研究成果: Article同行評審

29 引文 斯高帕斯(Scopus)

摘要

Fluorescent labelling and chromogenic reactions that are commonly used in conventional immunoassays typically utilize diffusion dominated transport of analytes, which is limited by slow reaction rates and long detection times. By integrating alternating current (AC) electrokinetics and electrochemical impedance spectroscopy (EIS), we construct an immunochip for rapid, sensitive, and label-free detection. AC electroosmosis (ACEO) and positive dielectrophoresis (DEP), induced by a biased AC electric field, can rapidly convect and trap the analyte onto an EIS working electrode within a few minutes. This allows the change of electron-transfer resistance (ΔRet) caused by the antibody-antigen (IgG-protein A) binding to be measured and quantified in real time. The measured impedance change achieves a plateau after electrokinetic concentration for only 90 s, and the detection limit is able to reach 200 pg ml-1. Compared to the conventional incubation method, the electrokinetics-enhanced method is approximately 100 times faster in its reaction time, and the detection limit is reduced by 30 times. The ΔR et of the positive response is two orders of magnitude higher than the negative control, demonstrating excellent specificity for practical applications.

原文English
頁(從 - 到)4656-4662
頁數7
期刊Analyst
138
發行號16
DOIs
出版狀態Published - 2013 八月 21

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 生物化學
  • 環境化學
  • 光譜
  • 電化學

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