A rapid paper-based detection system for determination of human serum albumin concentration

研究成果: Article

9 引文 (Scopus)

摘要

A rapid analytical platform composed of a paper-based chip (µPB-Chip) and a small analytic box is developed to determine the concentration of human serum albumin (HSA). In this study, a Bromocresol Green (BCG) reagent is dropped into the reaction area of the µPB-Chip, and the HSA concentration was derived from the R (red) value intensity of the albumin-BCG complex formed after a reaction process at 37 °C for 12 min. The effectiveness of the developed method is displayed using albumin concentrations ranging from 0 to 5 g/dL in HSA control samples. The measurement results acquired from 36 volunteer patient's HSA samples are presented to be in good consistency with those obtained utilizing a conventional assay technique (R2 = 0.9912). Ultimately, the results show that the developed platform provides a convenient, economical and dependable method for HSA concentration detection.

原文English
頁(從 - 到)241-246
頁數6
期刊Chemical Engineering Journal
352
DOIs
出版狀態Published - 2018 十一月 15

指紋

Serum Albumin
serum
Assays
Bromcresol Green
Albumins
detection
paper
assay
method

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

引用此文

@article{60fda5d6c40041359cb46b7f34ac176a,
title = "A rapid paper-based detection system for determination of human serum albumin concentration",
abstract = "A rapid analytical platform composed of a paper-based chip (µPB-Chip) and a small analytic box is developed to determine the concentration of human serum albumin (HSA). In this study, a Bromocresol Green (BCG) reagent is dropped into the reaction area of the µPB-Chip, and the HSA concentration was derived from the R (red) value intensity of the albumin-BCG complex formed after a reaction process at 37 °C for 12 min. The effectiveness of the developed method is displayed using albumin concentrations ranging from 0 to 5 g/dL in HSA control samples. The measurement results acquired from 36 volunteer patient's HSA samples are presented to be in good consistency with those obtained utilizing a conventional assay technique (R2 = 0.9912). Ultimately, the results show that the developed platform provides a convenient, economical and dependable method for HSA concentration detection.",
author = "Yang, {Ruey Jen} and Tseng, {Chin Chung} and Ju, {Wei Jhong} and Wang, {Hsiang Li} and Fu, {Lung Ming}",
year = "2018",
month = "11",
day = "15",
doi = "10.1016/j.cej.2018.07.022",
language = "English",
volume = "352",
pages = "241--246",
journal = "Chemical Engineering Journal",
issn = "1385-8947",
publisher = "Elsevier",

}

TY - JOUR

T1 - A rapid paper-based detection system for determination of human serum albumin concentration

AU - Yang, Ruey Jen

AU - Tseng, Chin Chung

AU - Ju, Wei Jhong

AU - Wang, Hsiang Li

AU - Fu, Lung Ming

PY - 2018/11/15

Y1 - 2018/11/15

N2 - A rapid analytical platform composed of a paper-based chip (µPB-Chip) and a small analytic box is developed to determine the concentration of human serum albumin (HSA). In this study, a Bromocresol Green (BCG) reagent is dropped into the reaction area of the µPB-Chip, and the HSA concentration was derived from the R (red) value intensity of the albumin-BCG complex formed after a reaction process at 37 °C for 12 min. The effectiveness of the developed method is displayed using albumin concentrations ranging from 0 to 5 g/dL in HSA control samples. The measurement results acquired from 36 volunteer patient's HSA samples are presented to be in good consistency with those obtained utilizing a conventional assay technique (R2 = 0.9912). Ultimately, the results show that the developed platform provides a convenient, economical and dependable method for HSA concentration detection.

AB - A rapid analytical platform composed of a paper-based chip (µPB-Chip) and a small analytic box is developed to determine the concentration of human serum albumin (HSA). In this study, a Bromocresol Green (BCG) reagent is dropped into the reaction area of the µPB-Chip, and the HSA concentration was derived from the R (red) value intensity of the albumin-BCG complex formed after a reaction process at 37 °C for 12 min. The effectiveness of the developed method is displayed using albumin concentrations ranging from 0 to 5 g/dL in HSA control samples. The measurement results acquired from 36 volunteer patient's HSA samples are presented to be in good consistency with those obtained utilizing a conventional assay technique (R2 = 0.9912). Ultimately, the results show that the developed platform provides a convenient, economical and dependable method for HSA concentration detection.

UR - http://www.scopus.com/inward/record.url?scp=85049438304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049438304&partnerID=8YFLogxK

U2 - 10.1016/j.cej.2018.07.022

DO - 10.1016/j.cej.2018.07.022

M3 - Article

AN - SCOPUS:85049438304

VL - 352

SP - 241

EP - 246

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

SN - 1385-8947

ER -