A rapid quantitative 1H NMR analysis of kinsenoside and other bioactive principles from: Anoectochilus formosanus

Li Ying Hsieh, Hsiu Hui Chan, Hsin Yi Hung, Chao Lin Kuo, Jin Bin Wu, I. Wen Sun, Ping Chung Kuo, Tian Shung Wu

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Anoectochilus formosanus is a famous traditional Taiwanese folk medicine. Kinsenoside (1), one of the major active components in A. formosanus, exhibits hepatoprotective, antihyperliposis and antiflammatory activities. In this study, a proton nuclear magnetic resonance (1H NMR) method was developed for rapid quantitative analysis of several bioactive major constituents, including kinsenoside (1), 3-(R)-3-β-d-gluco-pyranosyloxy-4-hydroxybutanoic acid (2), 1-O-isopropyl-β-d-gluco-pyranoside (3), 4-(β-d-gluco-pyranosyloxy)benzyl alcohol (4) and benzoic acid (5) in plant samples and commercial products of A. formosanus. Interferences were removed by a simple Diaion HP-20 column chromatography. In the 1H NMR spectrum, the signals of compounds 1 (δ 2.88), 2 (δ 2.47), 3 (δ 1.12), 4 (δ 7.05) and 5 (δ 7.84) were selected and integrated as the target peaks, separated well from other signals in D2O. The developed 1H NMR method provides a rapid and simple quantitative method without any derivatization, pH control or addition of NMR shift reagent, and the analytical solvent system is very safe, convenient and simple.

原文English
頁(從 - 到)5645-5650
頁數6
期刊Analytical Methods
8
發行號28
DOIs
出版狀態Published - 2016 7月 28

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 化學工程 (全部)
  • 工程 (全部)

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