A rapid screening for hemoglobin-specific aptamers by using a continuous microfluidic system

Ching Chu Wu, Hsin I. Lin, Chen Hsun Weng, Shu Chu Shiesh, Gwo Bin Lee

研究成果: Conference contribution

3 引文 斯高帕斯(Scopus)

摘要

Systematic evolution of ligands by exponential enrichment (SELEX) is a method to screen specific DNA- or RNA-based aptamers by repeated rounds of partition and amplification from random pool of oligonucleotides. The screening products have high binding affinity for the target molecule, which can be an excellent biomarker for diagnosis and therapeutics. This study reports a new continuous microfluidic system to perform SELEX in an automatic mode, which is an excellent tool for fast selection of aptamers. As a demonstration, hemoglomin-specific aptamer was screened. The screened aptamer can be used for fast diagnosis of hemoglobin, which is a crucial indicator for diabetes mellitus.

原文English
主出版物標題Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
發行者Chemical and Biological Microsystems Society
頁面353-355
頁數3
ISBN(列印)9780979806452
出版狀態Published - 2012 一月 1
事件16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
持續時間: 2012 十月 282012 十一月 1

出版系列

名字Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
國家/地區Japan
城市Okinawa
期間12-10-2812-11-01

All Science Journal Classification (ASJC) codes

  • 化學工程(雜項)
  • 生物工程

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