A reference map and identification of porcine testis proteins using 2-DE and MS

San Yuan Huang, Jyh Hung Lin, Yu Huey Chen, Chin Kai Chuang, En Chung Lin, Mu Chiou Huang, Hsiao Fang Sunny Sun, Wen Chuan Lee

研究成果: Article同行評審

49 引文 斯高帕斯(Scopus)

摘要

The development of the testis is essential for maturation of male mammals. A complete understanding of proteins expressed in the testis will provide biological information on many reproductive dysfunctions in males. The purposes of this study were to apply a proteomic approach to investigating protein composition and to establish a 2-D PAGE reference map for porcine testis proteins. MALDI-TOF MS was performed for protein identification. When 1 mg of total proteins was assayed by 2-D PAGE and stained with colloidal CBB, more than 400 proteins with a pI of pH 3-10 and Mr of 10-200 kDa could be detected. Protein expression varied among individuals, with CV between 4.7 and 131.5%. A total of 447 protein spots were excised for identification, among which 337 spots were identified by searching the mass spectra against the NCBInr database. Identification of the remaining 110 spots was unsuccessful. A 2-D PAGE-based porcine testis protein database has been constructed on the basis of the results and will be published on the WWW. This database should be valuable for investigating the developmental biology and pathology of porcine testis.

原文English
頁(從 - 到)4205-4212
頁數8
期刊Proteomics
5
發行號16
DOIs
出版狀態Published - 2005 11月

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 分子生物學

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